Liquid phase chip reagent box for early diagnosing mammary cancer and preparation method thereof

A technology of early diagnosis and liquid phase chip, which is applied in the field of medicine and biology, can solve problems such as damage, achieve low cost, simple and reliable preparation method, and good stability

Inactive Publication Date: 2008-06-18
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results have shown that the detection level of serum TNF in patients with breast cancer is significantly higher than that in the normal control group (p<0.01), thus indicating that TNF is involved in the occurrence and development of breast cancer, leading to pathological body damage

Method used

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  • Liquid phase chip reagent box for early diagnosing mammary cancer and preparation method thereof
  • Liquid phase chip reagent box for early diagnosing mammary cancer and preparation method thereof
  • Liquid phase chip reagent box for early diagnosing mammary cancer and preparation method thereof

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Embodiment 1

[0059] The IL-6 capture antibody is a monoclonal antibody to IL-6, the clone number is 6708; the IL-8 capture antibody is a monoclonal antibody to IL-8, the clone number is 6217.111, and the VEGF capture antibody is a monoclonal antibody to VEGF, the clone number 26503.111, T-PSA capture antibody is T-PSA monoclonal antibody, clone number is BM215, TNF-α capture antibody is TNF-α monoclonal antibody, clone number is 28401.111.

[0060] The IL-6 detection antibody is a polyclonal antibody to IL-6, the clone number is SV126053; the IL-8 detection antibody is a polyclonal antibody to IL-8, the clone number is OM096021, and the VEGF detection antibody is a polyclonal antibody to VEGF, the clone number It is XQ096031, the T-PSA detection antibody is the monoclonal antibody of T-PSA, the clone number is M701042, and the TNF-α detection antibody is the TNF-α monoclonal antibody, the clone number is D2.

[0061] In the present embodiment, the formula of described various solutions is ...

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Abstract

The invention discloses a liquid phase chip kit used for early diagnosis of breast cancer, which primarily comprises: (1) 5-plex coated microsphere comprising a No.51 microsphere coated with an IL-6 capture antibody, a No. 38 microsphere coated with an IL-8 capture antibody, a No. 34 microsphere coated with a TNF-Alpha capture antibody, a No. 20 microsphere coated with a T-PSA capture antibody, a No. 32 microsphere coated with a VEGF capture antibody; (2) intermixture to detect antibodies, which is marked with biomarkers of IL-6, IL-8; TNF-Alpha, T-PSA and VEGF; (3) streptavidin phycoerythrin. The invention discloses a method to prepare the liquid phase chip kit. The liquid phase chip kit used for early diagnosis of breast cancer has the advantages of no side effect, high flux, multiple indices used for parallel detection, high sensitivity, high repetitiveness, quick and accurate detection, low cost and so on.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a liquid phase chip kit for early diagnosis of breast cancer and a preparation method thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, with more than one million new cases every year in the world, most of which occur in women between the ages of 40 and 60, that is, women before and after menopause. The incidence of breast cancer in the world is increasing at an annual rate of 0.2-3%. In Western developed countries such as Europe and the United States, breast cancer has become one of the main causes of death for women, and one out of every 9 to 10 women suffers from breast cancer. In China, breast cancer is the most common malignant disease among Chinese women, accounting for 22% of malignant tumors and showing a rapid upward trend year by year. When the cancer is diagnosed before it has spread, the survival r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/546
Inventor 许嘉寿彭臻菲林一群
Owner SUREXAM BIO TECH
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