Method for separating, purifying and extracting fat mesenchymal stem cell

Abstract

The invention relates to the biological cell extract and application field and provides a method for separating, purifying and extracting mesenchymal stem cell in the fat, which comprises the following steps: first, extracting fat source under sterile condition, soaking by buffer, rinsing, adopting nutrient solution to do water-bath oscillation and digestion, fetching the sieved digestion material to do centrifugal disposal, obtaining base microtubule layer, sedimenting and removing erythrocyte, using nutrient solution to centrifugal and rinse after sieving the digestion material, using physiological saline to dilute the digested and separated cell, separating electrophoresis tank into three parts transversely by nylon screen cloth evenly, placing potassium chloride and calcium gluconate solution according to certain bulk rate, inputting AC power for 2-5 min, placing the obtained cell in the middle electrophoresis tank to stir evenly, electrophorezing for 15-30 min under DC power, extracting cell liquid in the electrophoresis tank at the positive electrode side, obtaining the mesenchymal stem cell in the fat. The method is short in period without causing gene sorting disorder or mutation on the DNA vortex chain.

Description

technical field [0001] The invention relates to the field of biological cell extraction and application, in particular to a stem cell extraction method. Background technique [0002] As one of the trends of future medicine, stem cell engineering is being paid attention by more and more scholars. On October 8, 2007, the Nobel Prize in Physiology or Medicine honored two American scientists and one British scientist for their outstanding contributions to stem cells. The main content is to use undifferentiated stem cells to modify specific genes in vivo through homologous DNA recombination, and lay the foundation for the development of "gene targeting" technology. This will produce a brand-new medical technology, which is to screen human genes and repair damaged tissues or organs through stem cells. [0003] Scientists in the United States have discovered that fat extracted from patients' buttocks and thighs contains a large number of stem cell-like cells that can develop into...

AI Technical Summary

Problems solved by technology

The main reason is that the stem cell extraction method is not easy to quickly yield. The current stem cell extraction method mainly adopts the traditional "adherence, culture, passage" method

The disadvantage of this method is that the cycle is too long, and the process of proliferation and replication of stem cells in vitro may easily cause gene sequence disorder or mutation on the DNA double-helical strand