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Test paper for detecting pig breeding and respiration syndrome antibody and method for making same

A technology for respiratory syndrome and pig reproduction, which is applied in the field of animal disease inspection and quarantine to achieve the effects of low production cost, timely control of epidemic situation, and simple and labor-saving operation.

Inactive Publication Date: 2008-07-09
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

The IFA method has high sensitivity, but it must have fluorescent immunomicroscope and darkroom conditions. In order to obtain a good fluorescence observation effect, it must be equipped with a suitable filter. The experimental time takes two hours. The results are detected under an immunological microscope, which must be performed by experienced professional technicians. Personnel Judgment Test Results

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  • Test paper for detecting pig breeding and respiration syndrome antibody and method for making same

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Embodiment 1

[0030] Embodiment 1 The present invention detects the preparation of porcine reproductive and respiratory syndrome antibody test strip

[0031] 1 Preparation of PRRSV N protein expressed by genetic engineering

[0032] ①Material source

[0033] (1) Virus strains and strains: PRRSV American strain ATCC-VR2332, expression vector, and recipient strain BL21 are all preserved by the Epidemiology and Diagnosis Center of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences; (2) Restriction enzyme EcoRI, XhoI, Taq enzyme, pET30(a), T 4 DNA ligase was purchased from Dalian Bao Biological Company; (3) IPTG and Kanamycin were purchased from Shanghai Sangong, and ProBord purification reagents and Trizol kits were purchased from Invitrogen.

[0034] ② Preparation of N protein

[0035] (1) The construction of the expression vector can be carried out with reference to the disclosed method of Zhou Yanjun et al. Application [J]. Chinese Journal of Preventive Veter...

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Abstract

The invention discloses a test paper for rapidly detecting antibodies against porcine reproductive and respiratory syndrome (PRRS) virus and a preparation method thereof. The inventive test paper consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by PRRSV M protein and a reference line (7) coated with rabbit anti PRRSV antibody; and the glass fiber member is combined with PRRSV N protein marked by colloidal gold. The test paper can be prepared in batches and has the advantages of simple process and low production cost. The test paper can be used for detecting porcine reproductive and respiratory syndrome without cultivating virus, thus achieving good safety. The inventive test paper has the advantages of high sensitivity, good reproducibility, simple, rapid and accurate operation and visual and easily-determined result.

Description

technical field [0001] The invention relates to a test strip for detecting animal diseases, in particular to a test strip for rapidly detecting porcine reproductive and respiratory syndrome antibodies. The invention also relates to a preparation method and application method of the test strip, which belongs to animal disease testing Quarantine field. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is a new disease that appeared in the late 1980s. Its symptoms are mainly manifested as abortion, premature birth, stillbirth, mummified fetus and post-infected piglets in pregnant sows. Decreased survival rate, respiratory symptoms in adult pigs, but also lead to immunosuppression and secondary to a variety of other diseases. At present, the disease is prevalent in almost all pig-raising areas in the world, causing huge economic losses to the world's pig-raising industry, and has aroused widespread ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/52G01N33/531
Inventor 崔尚金
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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