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Filter paper sheet diaphragmatic foramen suspension spore ejection method

A spore ejection method and filter paper technology, which are applied in the methods of using spores, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult transfer, easy pollution, and complicated operation, so as to reduce the time and pollution of pollution. the area and the effect of reducing bacterial contamination

Inactive Publication Date: 2008-07-16
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole mushroom insertion method requires a special device to collect the spores and dilute the coating, which is very cumbersome to operate
The hook suspension method is to hang the cap of the mushroom to make the spores fall into the culture medium of the triangular flask, which is easy to pollute and difficult to transfer
The attachment method is to attach the gills or caps to the slope or the cover of the petri dish, and after the spores fall, the culture medium is moved to a test tube or a petri dish for cultivation. The operation is cumbersome and easy to infect bacteria.

Method used

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  • Filter paper sheet diaphragmatic foramen suspension spore ejection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Prepare potato culture medium (PDA), sterilize it into a flat plate with a thickness of 0.5cm, and process a circular filter paper hole with a diameter of Φ0.5cm on the filter paper to prepare a perforated filter paper, which is sterilized by ultraviolet light spare;

[0018] 2. In the ultra-clean workbench, remove the lid of the petri dish, place the sterile perforated filter paper on the lower dish with the medium plate, and then collect the large fungus Collybia dryophila (Bull.Ex) from the field. Fr.)Que`l] fruiting body block is placed on the filter paper hole of a sterile perforated filter paper, after standing for 60 minutes, the filter paper and fruiting body are removed, the petri dish lid is covered, and it is moved to a 22°C incubator for constant temperature culture ;

[0019] 3. After the spores germinate, pick the mycelium and transfer it to the slope of the test tube to continue to cultivate until the tube is full, and further morphological identification ...

Embodiment 2

[0021] 1. Prepare potato culture medium (PDA), sterilize it into a flat plate with a thickness of 0.5cm, and process a circular filter paper hole with a diameter of Φ2cm on the filter paper sheet to prepare a perforated filter paper sheet, which is sterilized by high-pressure humid heat for use ;

[0022] 2. In the ultra-clean workbench, remove the lid of the petri dish, place the sterile perforated filter paper on the lower dish with the medium plate, and then put the large fungus Pleurotus ostreatus (Jacq.Ex) collected in the field. Fr.)Que`l] fruit body block is placed on the filter paper hole of a sterile perforated filter paper, after standing for 20 minutes, remove the filter paper and fruit body, cover the petri dish cover, and move to a 26°C incubator for constant temperature culture ;

[0023] 3. After the spores germinate, pick the mycelium and transfer it to the inclined surface of the test tube to continue to cultivate until the tube is full, and then perform morpholog...

Embodiment 3

[0025] 1. Prepare potato culture medium (PDA), sterilize it into a flat plate with a thickness of 0.5cm, and process a circular filter paper hole with a diameter of Φ1cm on the filter paper sheet to prepare a perforated filter paper sheet, which is sterilized by ultraviolet light for use;

[0026] 2. In the ultra-clean workbench, remove the lid of the petri dish, put the sterile perforated filter paper on the bottom dish with the culture plate, and then stick the large fungi collected in the field to the ears [Crepidotusmollis(Fr.)Staud] The fruiting body block is placed on the filter paper hole of a sterile perforated filter paper. After standing for 40 minutes, the filter paper and fruiting body are removed, and the petri dish lid is covered, and then moved to a 24℃ incubator for constant temperature bring up;

[0027] 3. After the spores germinate, pick the mycelium and transfer it to the slope of the test tube to continue to cultivate until the tube is full, and further morpho...

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Abstract

The invention discloses a filter paper separated hole suspended spore ejection method, which firstly prepares a sterile potato culture medium slab with the thickness of 0.5cm and processes a sterile filter paper which is provided with a hole with the diameter of Phi 0.5 to 2 cm; then a culture dish cover is removed from a super clean workbench, and the sterile filter paper with the hole is arranged on a lower dish which is provided with the culture medium slab; a large-scale fungal sporophore block which is collected at the field is arranged on the filter paper hole of the sterile filter paper with the hole for still placement for 20 to 60min, then the filter paper and the sporophore are removed, the culture dish cover is covered, and a constant temperature culture is carried out after transferring the fungal sporophore block into a culture box at 22 - 26 DEG C; finally, the mycelia are selected after spore germination and transferred into the slope of a test tube for continual culture till the tube is full; the configuration identification is further carried out, so as to obtain a wild large-scale fungal pure culture. The invention has the advantages of reducing the opportunity of hybrid bacteria pollution, improving the success rate of the obtainment of large-scale fungal pure culture and having simplified operation.

Description

Technical field [0001] The invention relates to the separation and purification of fungal strains, and in particular to a method for ejecting spores from a filter paper sheet, which can be efficiently and conveniently separated to obtain a pure culture of a large fungus. technical background [0002] The isolation of pure cultures of large fungi is directly related to the acquisition of the mother species, the quality of the mother species, and breeding materials. The spore ejection method is suitable for the separation of large fungi in a wide range and can obtain pure cultures of single spores. However, the spore ejection method is easy to contaminate the miscellaneous bacteria such as penicillium or bacteria. In addition, such miscellaneous bacteria and larger fungi multiply quickly and often lead to the failure of pure culture. There are three conventional spore ejection methods: whole mushroom planting method, hooking method and sticking method. The whole mushroom planting m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00
Inventor 霍光华陈明辉李红
Owner JIANGXI AGRICULTURAL UNIVERSITY
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