Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting polysaccharide from angelica and application thereof

A technology of angelica and polysaccharides, applied in the field of medical medicine, can solve the problems of preventing or treating osteoarthritis without applying polysaccharides, and treating osteoarthritis without using angelica, and achieving the effects of long duration of drug effect, easy implementation, and high yield

Active Publication Date: 2008-08-06
广州南峰生物科技有限公司
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant report on the use of Angelica sinensis alone in the treatment of osteoarthritis, and there is no report on the use of polysaccharides extracted from Angelica sinensis to prevent or treat osteoarthritis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the method for extracting active Angelica sinensis polysaccharide from Angelica sinensis

[0036] A, the dried Angelica sinensis is powdered into 80~100 mesh powder with pulverizer;

[0037] B. Put the powder into the extraction tank, add 3 times (W(g) / V(mL)) of petroleum ether (boiling range: 60-90°C) and reflux for 30-60 minutes to degrease, repeat this step twice, and filter out the petroleum Ether, obtain the residue after degreasing;

[0038] C. Add 7 (W(g) / V(mL)) times distilled water to the residue and extract under normal pressure at 100°C for 1 hour, boil and extract, repeat this step three times, and combine the three extracts;

[0039] D. Concentrate the extract under reduced pressure at 50°C, so that the volume of the solution (mL) is 5 times the weight (g) of the Angelica sinensis powder used in step A, to obtain a concentrated solution;

[0040] E. Add 3 times the volume of 95% ethanol to the concentrated solution in step D, and let it stand ...

Embodiment 2

[0044] Embodiment 2: the method for extracting active Angelica sinensis polysaccharide from Angelica sinensis

[0045] A, the dried Angelica sinensis is powdered into 80~100 mesh powder with pulverizer;

[0046] B. Put it into the extraction tank, add 4 times (W(g) / V(mL)) of petroleum ether (boiling range: 60-90°C) to reflux for 35 minutes to degrease, repeat this step twice, filter out the petroleum ether, and obtain degreasing residue after

[0047] C. Add 8 (W(g) / V(mL)) times distilled water to the residue and extract at 100°C under normal pressure for 1.5h, boil and extract, repeat this step three times, and combine the three extracts;

[0048] D. Concentrate the extract under reduced pressure at 55°C so that the volume of the solution (mL) is 6 times the weight (g) of the Angelica sinensis powder used in step A to obtain a concentrated solution;

[0049] E. Add 3 times the volume of 95% ethanol to the concentrated solution in step D, and let it stand overnight so as to ...

Embodiment 3

[0053] Embodiment 3: the method for extracting active Angelica sinensis polysaccharide from Angelica sinensis

[0054] A, the dried Angelica sinensis is powdered into 80~100 mesh powder with pulverizer;

[0055] B. Put it into the extraction tank, add 5 times (W(g) / V(mL)) of petroleum ether (boiling range: 60-90°C) to reflux for 40 minutes to degrease, repeat this step twice, filter out the petroleum ether, and obtain degreasing residue after

[0056] C. Add 8 (W(g) / V(mL)) times distilled water to the residue and extract at 100°C under normal pressure for 2 hours, boil and extract, repeat this step three times, and combine the three extracts;

[0057] D. Concentrate the extract under reduced pressure at 60°C, so that the volume of the solution (mL) is 7 times the weight (g) of the Angelica sinensis powder used in step A, to obtain a concentrated solution;

[0058] E. Add 3 times the volume of 95% ethanol to the concentrated solution in step D, and let it stand overnight so a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Maximum tolerated doseaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide. The invention further discloses an application of the active chiretta polysaccharide in the ostarthritis prevention or treatment drug preparation. The invention has definite effective components, definite curative effect, non toxic side effect and easy process.

Description

technical field [0001] The invention relates to the technical field of medical medicine, and more specifically relates to a method for extracting active polysaccharides from Angelica sinensis, and also relates to the use of the active polysaccharides extracted from Angelica sinensis in anti-osteoarthritis drugs. Background technique [0002] Articular cartilage tissue is mainly composed of chondrocytes and extracellular matrix (ECM), and the main components of ECM are type II collagen, proteoglycan aggregates and water. The degradation and repair of normal articular cartilage matrix are in a continuous dynamic equilibrium state, which is maintained by the secretion of cartilage matrix components by chondrocytes. Although the mechanism of progressive destruction and loss of articular cartilage in primary osteoarthritis is unclear, it has been recognized that osteoarthritis is characterized by metabolic disturbance and degradation of articular carti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61K31/715A61P19/02
Inventor 汪晖陈廖斌商亮谭扬李小军
Owner 广州南峰生物科技有限公司