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Production of acetone and butanol by sugar grass straw or/and sweet corn straw fermentation

A technology for sweet sorghum straw and sweet corn, which is applied in the field of using sweet sorghum straw or/and sweet corn straw to ferment and produce acetone and butanol

Inactive Publication Date: 2008-08-13
李世杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of sweet sorghum stalk juice to produce fuel ethanol has been widely reported at home and abroad, but the production of acetone and butanol by fermentation of sweet sorghum stalk juice has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Production of Acetone and Butanol by Fermentation of Sweet Sorghum Straw

[0026] 1. The production steps include:

[0027] Juicing of sweet sorghum stalks: 18kg of sweet sorghum stalks provided by Hubei Academy of Agricultural Sciences Grain Crop, squeezed by WY-807 small juicer to obtain 9000ml of straw juice with a sugar content of 17.0°Brix,

[0028] 2. Preparation of fermentation medium: Dilute the above-mentioned part of the straw juice to obtain straw juice with a sugar content of 7.0°Brix, and add the following substances according to the ratio of (mass / volume percentage): 0.3% corn steep liquor, CaCO 3 0.8%, (NH 4 ) 2 SO 4 0.5%, KH 2 PO 4 0.02%, adjusted to pH 6.7 with sodium hydroxide, distributed in 15 500ml Erlenmeyer flasks, sterilized at 121°C for 30 minutes, and used as a fermentation medium for subsequent use.

[0029] 3. Seed preparation:

[0030] Commercially available corn flour is passed through an 80-mesh sieve, adjusted to a slurry with 5%...

Embodiment 2

[0038] Production of Acetone and Butanol by Fermentation of Sweet Sorghum Straw

[0039] 1. The production steps include:

[0040] Juicing of sweet sorghum stalks: 18kg of sweet sorghum stalks provided by Hubei Academy of Agricultural Sciences Grain Crop, squeezed by WY-807 small juicer to obtain 9000ml of straw juice with a sugar content of 17.0°Brix,

[0041] 2. Preparation of fermentation medium: Dilute the above-mentioned part of the straw juice to obtain straw juice with a sugar content of 7.0°Brix, and add the following substances according to the ratio (mass / volume percentage): 0.6% corn steep liquor, CaCO 3 1.0%, (NH 4 ) 2 SO 4 1.0%, KH 2 PO 4 0.06%, adjust the pH to 6.8 with sodium hydroxide, pack in 15 500ml Erlenmeyer flasks, sterilize at 121°C for 30 minutes, and use it as a fermentation medium for future use.

[0042] 3, seed preparation: with embodiment 1.

[0043] 4, inoculation fermentation: with embodiment 1.

[0044] 5. Solvent detection:

[0045] ...

Embodiment 3

[0048] Production of Acetone and Butanol by Fermentation of Sweet Sorghum Straw

[0049] 1. The production steps include:

[0050] Juicing of sweet sorghum stalks: 18kg of sweet sorghum stalks provided by Hubei Academy of Agricultural Sciences Grain Crop, squeezed by WY-807 small juicer to obtain 9000ml of straw juice with a sugar content of 17.0°Brix,

[0051] 2. Preparation of fermentation medium: Dilute the above-mentioned part of the straw juice to obtain straw juice with a sugar content of 7.0°Brix, and add the following substances according to the ratio of (mass / volume percentage): 0.5% corn steep liquor, CaCO 3 1.0%, (NH 4 ) 2 SO 4 0.8%, KH 2 PO 4 0.04%, adjusted to pH 6.8 with sodium hydroxide, distributed in 15 500ml Erlenmeyer flasks, sterilized at 121°C for 30 minutes, and used as a fermentation medium for subsequent use.

[0052] 3, seed preparation: with embodiment 1.

[0053] 4, inoculation fermentation: with embodiment 1.

[0054] 5. Solvent detection:...

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Abstract

A method for preparing acetone-butanol by using sweet sorghum straw and sweet corn straw comprises the steps extracting juice from sweet sorghum straw and sweet corn straw and diluting sugar content of juice to 10-6, adding phosphates, calcium salt, nitrogen source and minim growth factor, sterilizing at up to 121 DEG C then cooling to 32-37 DEG C to obtain fermentation medium, inoculating acetone-butanol production strain in corn culture medium, heat activating at 100 DEG C and anaerobic cultivation at 37 DEG C to obtain seed liquor, accessing the seed liquor into fermentation medium according to fermentation medium volume ratio 2-5, anaerobic fermentation at 32-37 DEG C to obtain acetone and butyl alcohol fermentation broth, at last testing the content of acetone and butyl alcohol. The feature of the invention is in one aspect utilize straw in season, in another aspect, can utilize different kinds lower sugar-content sweet sorghum straw and sweet corn straw cultured in different areas and different season. The problem of conserving fresh straw for fermenting and preparing in large scale is settled.

Description

technical field [0001] The invention relates to a method for producing chemical products by using agricultural waste stalks for microbial conversion, in particular to a method for producing acetone and butanol by fermenting sweet sorghum stalks or / and sweet corn stalks. Background technique [0002] Acetone and butanol are important organic solvents and chemical raw materials, widely used in spray paint, explosives, plastics, pharmaceuticals, plant extraction, organic glass, synthetic rubber and other industries, among which butanol is more valuable . They can be produced by chemical synthesis and microbial fermentation. The raw material of chemical synthesis comes from petroleum. In recent years, the price of petroleum has risen sharply, and its resources are non-renewable. Chemical synthesis will eventually be replaced by fermentation. The microbial fermentation method can use agricultural and sideline products as raw materials, the process equipment is simple, the inves...

Claims

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Application Information

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IPC IPC(8): C12P7/28C12P7/16C12R1/01
Inventor 李世杰
Owner 李世杰
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