Application of anemarrhena asphodefoides extract in preparing medicaments for improving insulin resistance

A technology of Anemarrhena extract and insulin resistance, which is applied in the fields of molecular biology and pharmacology, can solve the problems of theoretical illusion and lack of molecular biology research support, and achieve the effect of improving insulin resistance

Inactive Publication Date: 2008-08-20
NANJING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Anemarrhena is the dried rhizome of Anemarrhena asphodeloides, a plant of the Liliaceae family. It has the effects of nourishing yin and reducing fire, moistening dryness

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of anemarrhena asphodefoides extract in preparing medicaments for improving insulin resistance

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example

[0018] 1. Concentration method of the extract of Anemarrhena serrata Add 1L of distilled water to 150g of Anemarrhena vulgaris, heat to boiling, decoct for 2 hours, save the supernatant, and repeat the above process for the residue. The two supernatants were transferred to a rotary evaporator at 60°C and evaporated for about 4 hours, then freeze-dried at low temperature; stored at 4°C.

[0019] 2. Mice administration and tissue isolation and preservation The experimental mice were SPF wild-type male mice, strain C57BL / 6, 8 weeks old, and were raised in the Animal Experimental Center of Nanjing Gulou Hospital. Gavage with a gavage needle at a dose of 90 mg / kg twice, with an interval of 8 hours; mice in the control group were gavaged with an equal volume of normal saline. The mice were sacrificed 6 hours after the second gavage; the mouse kidney tissues were taken, rinsed with normal saline, and immediately placed in a cryopreservation tube, and stored in liquid nit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for lavaging the stomach of a mouse by use of rhizoma anemarrhenae aqueous extract, belonging to the technical fields of molecular biology and pharmacology, which is characterized in that: by means of the specific inhibition of the expression of uncoupling protein 2 (UCP 2), the insulin resistance can be overcome. The method in the invention comprises the following steps: decocting rhizoma anemarrhenae with water, then condensing and freeze-drying the decocted rhizoma anemarrhenae by means of pressure reduction, and finally obtaining the crystal condensed extract. When the extract is used to prepare aqueous solution with a certain concentration, and the aqueous solution is used to lavage the stomach of a mouse, the expression of UCP 2 in the kidney of the mouse can be remarkably inhibited. Furthermore, the invention explains the fact that rhizoma anemarrhenae aqueous extract can suppress the expression of UCP2 in the kidneys in a specific manner, and therefore can be developed into a drug for improving and curing insulin resistance.

Description

1. Technical field [0001] The invention belongs to the technical fields of molecular biology and pharmacology. 2. Technical Background [0002] Insulin resistance refers to the decreased sensitivity of target cells to insulin, requiring more insulin to maintain normal blood sugar concentration, which is manifested as the impairment of glucose utilization by peripheral tissues, especially muscle and adipose tissue. In the early stage of the disease, the pancreatic β cells can increase the secretion of insulin to make up for its insufficient effect. However, with the gradual failure of the pancreatic β cell function, that is, the loss of the compensatory mechanism, insulin resistance, hyperinsulinemia, and hyperinsulinemia will appear. High blood sugar and so on then lead to diabetes. Modern medicine has shown that insulin resistance is a common risk factor for many cardiovascular diseases and diabetes, and can cause a variety of metabolic-related diseases. [0003] UCP2 is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/9064A61P5/50A61K125/00
Inventor 姜晓宏张辰宇项阳孙凌冰张红杰孙国勋邹季虹徐琛
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap