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Application of anemarrhena asphodefoides extract in preparing medicaments for improving insulin resistance

A technology of Anemarrhena extract and insulin resistance, which is applied in the fields of molecular biology and pharmacology, can solve the problems of theoretical illusion and lack of molecular biology research support, and achieve the effect of improving insulin resistance

Inactive Publication Date: 2008-08-20
NANJING UNIV
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Problems solved by technology

[0004] Anemarrhena is the dried rhizome of Anemarrhena asphodeloides, a plant of the Liliaceae family. It has the effects of nourishing yin and reducing fire, moistening dryness and smoothing the intestines. Diseases are caused by the imbalance of cold and heat, but these theories are too illusory and lack the support of modern molecular biology research

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  • Application of anemarrhena asphodefoides extract in preparing medicaments for improving insulin resistance

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Embodiment

[0018] 1. Concentration method of Anemarrhena extract Add 1L of distilled water to 150g Anemarrhena, heat to boiling, decoct for 2 hours, keep the supernatant, and repeat the above process once for the residue. The two supernatants were transferred to a rotary evaporator at 60°C for about 4 hours, then freeze-dried at low temperature; stored at 4°C.

[0019] 2. Administration of mice and separation and storage of tissues The mice used in the experiments were wild-type male mice of SPF grade, strain C57BL / 6, 8 weeks old, and were bred in the Animal Experiment Center of Nanjing Drum Tower Hospital. The dose of 90 mg / kg was administered by intragastric administration twice with an interval of 8 hours; the mice in the control group were intragastrically administered with an equal volume of normal saline. The mice were killed 6 hours after the second gavage; the kidney tissues of the mice were taken, rinsed with normal saline, immediately placed in cryopreservation tubes, and store...

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Abstract

The invention relates to a method for lavaging the stomach of a mouse by use of rhizoma anemarrhenae aqueous extract, belonging to the technical fields of molecular biology and pharmacology, which is characterized in that: by means of the specific inhibition of the expression of uncoupling protein 2 (UCP 2), the insulin resistance can be overcome. The method in the invention comprises the following steps: decocting rhizoma anemarrhenae with water, then condensing and freeze-drying the decocted rhizoma anemarrhenae by means of pressure reduction, and finally obtaining the crystal condensed extract. When the extract is used to prepare aqueous solution with a certain concentration, and the aqueous solution is used to lavage the stomach of a mouse, the expression of UCP 2 in the kidney of the mouse can be remarkably inhibited. Furthermore, the invention explains the fact that rhizoma anemarrhenae aqueous extract can suppress the expression of UCP2 in the kidneys in a specific manner, and therefore can be developed into a drug for improving and curing insulin resistance.

Description

1. Technical field [0001] The invention belongs to the technical fields of molecular biology and pharmacology. 2. Technical Background [0002] Insulin resistance refers to the decreased sensitivity of target cells to insulin, requiring more insulin to maintain normal blood sugar concentration, which is manifested as the impairment of glucose utilization by peripheral tissues, especially muscle and adipose tissue. In the early stage of the disease, the pancreatic β cells can increase the secretion of insulin to make up for its insufficient effect. However, with the gradual failure of the pancreatic β cell function, that is, the loss of the compensatory mechanism, insulin resistance, hyperinsulinemia, and hyperinsulinemia will appear. High blood sugar and so on then lead to diabetes. Modern medicine has shown that insulin resistance is a common risk factor for many cardiovascular diseases and diabetes, and can cause a variety of metabolic-related diseases. [0003] UCP2 is ...

Claims

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Application Information

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IPC IPC(8): A61K36/9064A61P5/50A61K125/00
Inventor 姜晓宏张辰宇项阳孙凌冰张红杰孙国勋邹季虹徐琛
Owner NANJING UNIV
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