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Mobile type diaphragm clamp basin with cell growth substrate coating

A patch clamp, movable technology, applied in the direction of biomass post-treatment, biomass pre-treatment, biochemical instruments, etc., can solve the problems of slow cell attachment speed, low cell attachment rate, and prolonged operation time, and achieve shortened The effect of experimental manipulation time

Inactive Publication Date: 2008-08-20
周晋 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of slow cell adhesion rate and low cell adhesion rate in the movable patch clamp bath during the experiment process, which significantly prolongs the experimental operation time, and provides a movable patch clamp bath with a cell growth matrix coating. Patch Clamp Bath

Method used

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  • Mobile type diaphragm clamp basin with cell growth substrate coating
  • Mobile type diaphragm clamp basin with cell growth substrate coating

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Experimental program
Comparison scheme
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specific Embodiment approach 1

[0009] Specific implementation mode one: refer to figure 1 with figure 2 , the present embodiment is made up of bath cup 1, bath cup cover 2 and bath cup holder 3, and bath cup holder 3 has the hole 5 that places bath cup 1 above, is coated with a layer of cell growth matrix coating 4 on the inner wall of bath cup 1 . This method can make the cells adhere to the wall and significantly shorten the experimental operation time.

specific Embodiment approach 2

[0010] Specific implementation mode two: refer to figure 2 , The difference between this embodiment and the first embodiment is that the cell growth matrix coating (4) is polylysine, and other compositions and connections are the same as the first embodiment. The use of poly-lysine coating can shorten the attachment time of acutely isolated cells to less than half an hour, the attachment time of adherent cells to less than 3 hours, and the complete attachment of suspension cells and shorten the attachment time to 10 hours within.

specific Embodiment approach 3

[0011] Specific implementation mode three: refer to figure 2 , The difference between this embodiment and the first embodiment is that the cell growth matrix coating (4) is fibronectin, and other compositions and connections are the same as those of the first embodiment. The use of fibronectin coating can shorten the time of acutely isolated cells to adhere to the wall to less than half an hour, the time to adhere to the wall of adherent cells to less than 4 hours, and the time to completely adhere to the wall of suspended cells and shorten the time to adhere to the wall to less than 12 hours.

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Abstract

A moving patch clamp bath with a cell growth matrix coating layer relates to the patch clamp bath for electrophysiology experiment. The patch clamp bath includes a bath cup 1, a bath cup cover 2 and a bath cup rack 3 to solve the problems of slow cell attachment and low cell attachment rate during the experimental process of the moving patch clamp bath and significantly prolong experimental operation time; a hole 5 to place the bath cup 1 is arranged at the upper surface of the bath cup rack 3, and the cell growth matrix coating layer 4 is coated on the inner wall of the bath cup 1. The moving patch clamp bath with the cell growth matrix coating layer of the invention has easy cell attachment, shortened experimental operation time, simple structure and easy processing and manufacture.

Description

technical field [0001] The invention relates to a patch clamp bath for electrophysiological experiments. Background technique [0002] The patch clamp technique is to use a micro-glass tube electrode to contact the cell membrane in the bath, and seal it with an impedance of more than gigaohms, so that the small area of ​​the cell membrane connected to the opening of the electrode tip is electrically insulated from its surroundings. A method of detecting and recording the ion current of the ion channel on the membrane at a fixed potential. Since the patch clamp technique was invented by Neher and Sakmann in 1976, it has been widely used in the study of ion channels in excitable cells and non-excitable cells. [0003] The patch clamp is mainly composed of a highly sensitive current-voltage converter, a silver needle in a micropipette, a patch clamp bath, and a reference electrode. Among them, the bath is the key component of the patch clamp to clamp the cells, form the circu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 周晋王巍肖建民
Owner 周晋