Method for measuring photocatalysis nano-active water and free radical and uses thereof
A determination method, a technology of free radicals, applied in chemical method analysis, material analysis by observing the effect on chemical indicators, measurement devices, etc. Lack of awareness and other problems to achieve the effect of improving immunity and prolonging life
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Embodiment 1
[0091] Dissolve two parts of 1 gram of copper sulfate analytically pure reagent into 1000 ml of photocatalytic nano-active water and the raw water of photocatalytic nano-active water used for production, stir well and place it for 24 hours at regular intervals, and precipitates will be produced after chemical reactions occur , use medium-speed quantitative filter paper to filter the precipitate, measure the weight of the precipitate after drying, and use the difference in the weight of the precipitate to determine the activity of the photocatalytic nano-active water. The reaction formula is as follows:
[0092] CuSO 4 +(H + )+(OH - ) Radical-Cu(HO) 2 Precipitation+H 2 SO 4
[0093] Cu(HO) 2 It is a blue precipitate, and the oxidation ability of photocatalytic nano-active water can be determined after collecting the precipitate, filtering, drying, and weighing. According to the reaction formula, the number of free radicals in photocatalytic nano-active water can be calc...
Embodiment 2
[0095] 1. Take 4.5g of nutrient agar, put it into a beaker of 100ml distilled water, and sterilize four 90mm petri dishes at the same time under high pressure.
[0096] 2. Put the sterilized nutrient agar into four 90mm Petri dishes. Cool for 15 minutes until the agar solidifies.
[0097] 3. Drop 1ml of photocatalytic nano-active water into a small area on the surface of solidified agar in two 90mm petri dishes respectively. Then drop an equal amount of distilled water into a small area on the solidified agar surface of two 90 mm Petri dishes.
[0098] 4. Put the culture dish into the incubator for 12 hours.
[0099] 5. Observe the traces of oxidative decomposition of the culture medium in the area where the active water is dripped. The results show that there are different oxidation traces in the area covered by active water, the oxidized area is light yellow, the area with less active water forms light yellow spot-like spots, and the area with more active water concentrat...
Embodiment 3
[0105] Take 0.1ml of the serum of a hepatitis B patient, add 1ml of grade 4 photocatalytic nano-active water, the color is light yellow, and the absorbance is 3.265. Put it at 37°C for 72 hours, the color is white, and the absorbance is 0.862.
[0106] Hepatitis B surface antigen (HBsAg) dropped from 3.265 to 0.862, less than 1, showing negative. It shows that the free radicals in photocatalytic nano-active water can oxidize and decompose hepatitis B virus.
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