Chinese jujube micro-grafting method under condition of tissue culture

Abstract

Healthy tissue culture seedlings of a scion variety (strain) are cut into bladeless stem segments of 2-3cm at length to be as scions, healthy tissue culture seedlings of a rootstock variety (strain) are cut into bladed stem segments of 2-3cm at length to be as rootstocks, and the stem segments are soaked and pretreated for 10m in a GA3 solution of 0.5-1.0mg / L in concentration; under an aseptic condition, split grafting is used for carrying out micro-grafting and tinfoil is used for binding grafting interfaces; micro-grafted seedlings are vertically inserted into the culture medium to culture, cotton plugs are used for sealing the bottle mouths of culture bottles and then monolayer plastic sealing membrane is covered and tightly bound; the basic culture medium of the micro-grafted seedlings is MS, wherein, the concentration of the sucrose is 50g / L, the pH value before sterilizing of the culture medium is 6.0 and the temperature of a culture chamber is 28 DEG C. The adopted micro-grafting method can improve the survival rate of the grafting to 83 percent, realize scaled rapid filtration of disease-resistant varieties and idioplasm and is beneficial to develop researches such as the relevant rapid testing of pathogen and virus elimination.

Description

technical field

[0001] The invention relates to a jujube micro-grafting method under tissue culture conditions. Background technique

[0002] Usually micrografting refers to the technique of grafting rootstock and scion under tissue culture conditions. Since Murashige created micro-bud grafting technology in 1972, micro-grafting technology has been widely used in the research and production of fruit trees such as apples, pears, grapes and citrus, mainly including rapid virus detection, fruit tree rejuvenation, virus-free rapid propagation and Early identification of grafting compatibility and other aspects.

[0003] Jujube witches'broom disease is the most common and serious infectious disease in jujube production. Breeding excellent disease-resistant varieties is the fundamental way to solve the problem of jujube witches'broom disease. The jujube madness research group of the China Jujube Research Center of Hebei Agricultural University has selected two highly resistant g...

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