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Method for extracting chlorogenic acid

A chlorogenic acid and concentration technology, applied in the separation/purification of carboxylate, pharmaceutical formulations, plant raw materials, etc., can solve the problems of organic solvent residues, complicated extraction and purification processes, etc., and achieve the effect of low production cost

Inactive Publication Date: 2011-05-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

More organic reagents are used in these patents, such as methanol (03111946.8; 200310111180.2), chloroform (200510000484.0), acetone, sherwood oil, ethyl acetate (patent application number 200510020706.5; 200410035758.5), etc., making the extraction and purification process more complicated, Easy to introduce residues of organic solvents

Method used

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  • Method for extracting chlorogenic acid

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Effect test

Embodiment 1

[0032] Embodiment 1, the method for extracting chlorogenic acid from artichoke leaves and its effect verification

[0033] Follow the steps below to extract chlorogenic acid from artichoke leaves:

[0034] 1. Artichoke leaves are dried in the air or dried with nitrogen, and crushed into artichoke dry leaf powder with a particle size of 20-40 meshes; 10 kg of artichoke dry leaf powder is weighed and added to 90 liters of ethanol solution with a concentration of 70% by volume, Stir and extract for 3 hours, filter, and collect the filtrate to obtain the first extract. The filter residue was extracted for the second time, and 40 liters of 70% ethanol solution was added according to 1 kg dry leaf powder of artichoke: 4 L of 70% ethanol solution by volume, and extracted for 2 hours. After the second extraction, the supernatant was sucked after static settling, the precipitate was filtered through a 100-mesh membrane to obtain a filtrate, and the filtrate and supernatant were combin...

Embodiment 2

[0039] Example 2, the method for extracting chlorogenic acid from artichoke leaves and its effect verification

[0040] Follow the steps below to extract chlorogenic acid from artichoke leaves:

[0041] 1. Weigh 10 kg of fresh artichoke leaves and add 20 liters of 95% ethanol solution by volume, and homogenize with a masher. After homogenization, the ethanol volume percentage concentration of the liquid phase is 68%, standing for 2 hours, stirring intermittently for 2-3 times, filtering, and collecting the filtrate to obtain the first extract. The filter residue was extracted for the second time, and the filter residue was mixed with the 70% ethanol solution according to the volume percentage concentration of 1kg filter residue: 1L of 70% ethanol solution, and stirred by a mixer for 30 minutes to obtain the second extraction solution. Combine the extracts from the first and second extractions to obtain ethanol extracts.

[0042] 2. Concentrate the ethanol extract obtained in...

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Abstract

The invention discloses an extraction method of chlorogenic acid, which comprises (1), mixing artichoke leaf with alcohol, extracting in liquid phase, while the volume percentage of alcohol is 60-80%, collecting liquid phase to obtain extract liquor, (2), adding clarifier into the extract liquor, deposting protein, tannin and resin impurities, and filtering to obtain filter liquor, (3), passing the filter liquor through a chromatography column whose stuff is macroporous resin, spraying water until the eluent has no color, using the alcohol solution whose pH value is 1-3 as eluting agent to elute, recovering the eluent containing chlorogenic acid, (4), concentrating the eluent containing chlorogenic acid to obtain the solid containing chlorogenic acid, and suspending and washing via the alcohol solution whose volume percentage is at least 95% and water, for 3-5 times. The purity of prepared chlorogenic acid can reach 90% at least with low production cost and the product has high safety.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid, in particular to a method for extracting chlorogenic acid from artichoke leaves. Background technique [0002] Artichokes are native to North Africa and Southern Europe along the Mediterranean coast. As a perennial herb, it was used to treat indigestion and protect the liver as early as ancient Rome and ancient Greece. The edible parts of the artichoke are mainly the receptacle and the base of the bud of the unopened flower. my country currently has large areas of cultivation in Yunnan and Zhejiang. The canned products of flower buds are mainly exported. With the further expansion of international trade, the production area and processing output of artichoke will further increase. The yield of leaves as a by-product will also be greatly increased, with an artichoke plant producing far more leaves than flower bulbs. And the current leaves are processed or discarded, or used as fodde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/48C07C69/732A61K36/28
Inventor 宋曙辉许勇张宝海王文琪何洪巨唐晓伟冯丽
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES