Sterilizing composition
A composition, carbendazim technology, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of ineffectiveness, reduction of drug control effect, adaptive variation of fungal strains, etc., to expand the bactericidal spectrum and reduce selectivity Chances of resistance, effects of delaying onset and progression
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Embodiment 1
[0068] Example 1. In vitro biological activity
[0069] The combined virulence of formulation example 1 (45% water dispersible granules) and formulation example 4 (35% suspension concentrate) to apple ringworm was determined by the mycelium growth rate test. Dilute the test agent to the designed concentration, take 1 ml and add it to a petri dish, then add 9 ml of PSA medium, shake well and prepare a plate containing the drug diluted 10 times to the final concentration. To add 1 ml of sterilized water as a blank control. Use a puncher with a diameter of 4 mm to take out the plate along the outer edge of the test flora, place it in the flat plate containing the drug, and incubate in the dark at 24±1°C for 72 hours, measure the expanded diameter of the plate for each treatment, and calculate the growth inhibition percentage of the bacteria. Calculate the effective intermediate concentration (EC) of the drug by the concentration logarithm-inhibition rate probability value method...
Embodiment 2
[0073] Embodiment 2. Greenhouse potted experiment (prevention and treatment of cucumber gray mold test result)
[0074] The test crops were cultivated in the greenhouse to the 2-3 leaf stage seedlings, sprayed the leaves of the seedlings according to the designed concentration, and inoculated with the test pathogenic bacteria 24 hours after the chemical treatment. Then the seedlings were kept moist and cultured at 2±1°C, and the result investigation was carried out after the blank control without pesticide application had fully developed disease. Disease classification and control effect calculation methods are carried out in accordance with the national standard of the People's Republic of China "Guidelines for Field Efficacy Tests of Pesticides". The test results show that the control effect of the composition of the present invention has obvious synergistic effect compared with the separate application of the two components. The test results are shown in Table 2
[0075] ...
Embodiment 3
[0077] Embodiment 3. field test (to the field efficacy test of rape sclerotinia)
[0078] The field efficacy test on Sclerotinia sclerotiorum was carried out in the land cultivation area, and the test field of rape showed a certain resistance to carbendazim single agent because it had been used for many years to control Sclerotinia sclerotiorum. The test agent is Example 1 (45% water dispersible granule). A total of 3 doses were set up in the test, repeated 4 times, and Shi Qingshui was used as a blank control. The experimental fields were all natural disease and sprayed at the early stage of disease. Disease investigation was carried out when the blank control was fully onset. Disease grading and control efficacy calculation methods are carried out in accordance with the national standard of the People's Republic of China "Guidelines for Field Efficacy Tests of Pesticides". The test results show that the composition of the invention has an excellent control effect on preve...
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