Snake venom cytotoxin, preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of cytotoxin and snake venom, applied in the field of snake venom cytotoxin and its preparation, to achieve the effect of light side effects
Active Publication Date: 2008-09-24
JINZHOU AHON PHARM CO LTD
View PDF0 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, the extraction of cytotoxins with anti-cancer effec...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] Embodiment 1, the preparation of snake venom cytotoxin
[0021] In this example, the lyophilized powder of viper venom (purchased from Jingshi Kaitai Pharmaceutical Co., Ltd.) was used as the raw material.
[0022] The extracted experimental steps are as follows:
[0023] 1. Snake venom dissolving: Dissolve 8 g of Viper saw scales lyophilized powder in 100 ml of Tris-HCl buffer solution (pH 8.2, 0.05 mol / L).
[0024] 2. Remove impurities: Add 6ml of saturated ammonium sulfate solution to the snake venom solution obtained in step 1, mix and place at room temperature for 1 hour, centrifuge at 3000rpm for 10 minutes, discard the precipitate, and keep the supernatant solution.
[0025] 3. Desalination by dialysis: seal all the supernatant obtained in step 2 in a dialysis bag with a molecular weight cut-off of 14000D, then put the dialysis bag into distilled water for dialysis for 15 hours, change the water every 3 hours, and the volume of distilled water is 1200ml. The di...
Embodiment 2
[0032] Example 2, Functional verification of viper venom cytotoxin
[0033] 1. Validation of anti-cancer function of viper venom cytotoxin
[0034] Taking the human laryngeal cancer cell line Hep-2 (provided by the Institute of Head and Neck Surgery, Liaoning Medical College) as the target cell, the efficiency comparison of the snake venom cytotoxin and pingyangmycin inducing the apoptosis of cancer cells was observed. The experimental method refers to the literature (Tai Jun Wang Xuefeng Pingyangmycin-induced apoptosis of human laryngeal carcinoma Hep-2 cells "Journal of Liaoning Medical College" 2004Aug.25 (4) 5-7 pages), as follows:
[0035] Cultivation of the human laryngeal carcinoma cell line Hep-2 cell line: the cells were inoculated in the complete culture medium of RPMI-1640 (fetal bovine serum 10%, penicillin 100u / ml, streptomycin 100ug / ml), placed at 37°C, containing 5%CO 2 Cultured in an incubator.
[0036] Take 20ml of the viper venom cytotoxin stock solution p...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
Molecular weight
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a snake poison cytotoxin, the preparation method and the application. The method of preparing the snake poison cytotoxin in the invention includes the following steps: firstly, the snake poison is conducted an ion-exchange chromatography and then the eluent between a first absorption peak and a second absorption peak on the absorbance curve with a detection wavelength of 280nm in the eluent is gathered; secondly, the eluent obtained in the first step is conducted an exclusion chromatography, water is used as a moving phase, and the second absorption peak on the absorbance curve with a detection wavelength of 280 nm in the eluent is gathered to obtain the snake poison cytotoxin. The saw-scale viper poison cytotoxin in the invention can induce the human laryngeal cancer cell Hep-2 to an apoptosis and the capability of inducing Cell Hep-2 to an apoptosis is stronger than Pingyangmycin with a viscosity of 100 Mug/ml when the snake poison cytotoxin has a concentration of 0.01 mg per milliliter. In addition, experiments of undue toxicity prove that the cytotoxin has slight side effects. The saw-scale viper poison cytotoxin in the invention can be used as an anti-cancer drug to turn biological anti-cancer drugs into a reality.
Description
technical field [0001] The invention relates to a snake venom cytotoxin and its preparation method and application. Background technique [0002] At present, the anticancer drugs used clinically are mainly chemical drugs, which are often called chemotherapy drugs. The common disadvantage of this class of drugs is serious side effects, which can generally cause severe vomiting and hair loss in patients. Therefore, biological anticancer drugs with less side effects have become a hot topic in research in recent years, mainly focusing on the research of snake venom and centipede toxin. [0003] The venom of some snake species contains cytotoxins that can destroy the structure of tumor cells. There have been related reports before. For example, cells with anti-cancer effects have been found in the venom of the American copperhead and the venom of the akistrodon viper in southern Anhui, my country. Toxins, these discoveries are still in the stage of academic research and have not...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more