Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its immunity detecting method

An immunosensor and array electrode technology, which is applied in the field of enzyme-catalyzed conductance immunosensor and immunodetection based on micro-gap array electrodes, can solve the problem of low sensitivity, and achieve the effects of high sensitivity, portable price, and simple preparation

Inactive Publication Date: 2008-09-24
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of low sensitivity of traditional immunoassay technology and the need for quantitative detection of precision instru

Method used

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  • Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its immunity detecting method
  • Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its immunity detecting method
  • Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its immunity detecting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Detection of human immunoglobulin G (hIgG) by an immunosensor based on micro-gap array electrodes.

[0035] 1. Preparation of immunosensor:

[0036] The micro-gap array gold electrode produced by the conventional photolithography printing method is used as the substrate, which is an interdigitated double electrode, and the positive and negative electrodes are two comb-shaped gold electrodes, which cross each other to form a micro-gap array gold electrode. Comb tooth width is 1μm-100μm, gap is 1μm-100μm;

[0037] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1 M NaOH solution for 30 min; then the electrode was washed three times with ultrapure water (1 min each time), and dried . Then, the electrode was immersed in an ethanol solution containing 5% (volume percentage) of aminopropyltrimethoxysilane, and stood at room temperature for 24 hours. After washing with ultra...

Embodiment 2

[0042] Example 2: Detection of prostate-specific antigen (PSA) by an immunosensor based on micro-gap array electrodes.

[0043] 1. Preparation of immunosensor:

[0044] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1 M NaOH solution for 30 min; then the electrode was washed three times with ultrapure water (1 min each time), and dried ; Then, immerse the electrode in an ethanol solution containing 5% (volume percent) of aminopropyltrimethoxysilane, and let it stand at room temperature for 24 hours. After washing with ultrapure water for three times and drying, a silanized micro-gap array gold electrode was obtained.

[0045] Immerse the silanized micro-gap array gold electrode in glutaraldehyde aqueous solution (5% mass fraction), and let it stand at room temperature for 1 hour; after washing with ultrapure water three times, add 30 μL mouse anti-human PSA monoclonal antibody solution ...

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Abstract

The invention provides an enzyme-catalyzed conductivity immune sensor based on a micro-gap array electrode and an immune detection method thereof, the immune sensor comprises a micro-gap array electrode, an electrode substrate which is carried out the silanization treatment and enzyme sediments with great conductivity; the invention utilizes the micro-gap electrode array immune sensor, fixes the antibody of the object to be detected on the electrode gap substrate and utilizes an enzyme-labeled antibody sandwich to carry out the detection of the protein to be detected. The method of the invention has the advantages of high sensitivity and simple operation, the device is portable and the price is cheap, which possibly provides the immune detection technology with rapidity, practicality, low cost, high sensitivity and high throughput for the early and on-site detection and application of the diseases.

Description

technical field [0001] The invention relates to the preparation of a micro-gap electrode array immunosensing device and its immunodetection method. Background technique [0002] Immunoassay is one of the most commonly used technologies for biomedical molecular detection. It is of great significance for the rapid screening and detection of pathogens (pathogens, viruses), disease markers, drugs and chemical biotoxins. It is a clinical diagnosis, It is a major research tool in the fields of medical research, food and public safety, drug and poison analysis, and environmental monitoring. Currently, the most commonly used immunoassay techniques are enzyme-linked immunoassay, fluorescence immunoassay, gold standard lateral flow immunochromatography, etc. The sensitivity of these detection methods is not high, and it is difficult to realize the diagnosis and research of early diseases, and most of these methods require the use of precision instruments for quantitative analysis, wh...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/535G01N33/53G01N27/327
Inventor 蒋健晖黄勇俞汝勤沈国励楚霞
Owner HUNAN UNIV
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