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Method for preparing high-purity salidroside from privet

A salidroside, high-purity technology, applied in the field of medicine, to achieve cost-saving, alleviate resource shortage, and high-purity effects

Inactive Publication Date: 2008-10-08
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no patent related to the preparation of high-purity salidroside from Ligustrum lucidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take the dried and mature fruit of Ligustrum lucidum of the genus Ligustrum in Oleaceae, crush it to coarse powder, add 10 times the amount of 75% ethanol to carry out reflux extraction for 3 times, each time is 1 hour, combine the extracts, and recover under reduced pressure at 60 ° C ethanol, vacuum-dried to obtain the alcohol extract containing salidroside. The ethanol extract was dispersed in water 10 times the amount of the crude drug, centrifuged at 4000 rpm for 30 minutes, and the supernatant was poured out, which was the loading stock solution. The loading stock solution was diluted to a concentration of 5.7mg / ml (calculated by total phenol content), the adsorption flow rate was 3BV / h, the diameter-to-height ratio of the resin column was 1:8, the sample volume was 5 mg / ml (calculated by total phenol content), washed with water Remove 10-15 times the resin volume for impurity removal, the impurity removal flow rate is 6BV / h, and 5 times the resin volume is eluted...

Embodiment 2

[0023] Take the dried and mature fruit of Ligustrum lucidum of the genus Ligustrum in Oleaceae, crush it to coarse powder, add 10 times the amount of 75% ethanol to carry out reflux extraction for 3 times, each time is 1 hour, combine the extracts, and recover under reduced pressure at 60 ° C ethanol, vacuum-dried to obtain the alcohol extract containing salidroside. The ethanol extract was dispersed in water 10 times the amount of the crude drug, centrifuged at 4000 rpm for 30 minutes, and the supernatant was poured out, which was the loading stock solution. The loading stock solution was diluted to a concentration of 5.7mg / ml (calculated by total phenol content), the adsorption flow rate was 3BV / h, the diameter-to-height ratio of the resin column was 1:8, the sample volume was 5 mg / ml (calculated by total phenol content), washed with water Remove 12.5 times the resin volume for impurity removal, the impurity removal flow rate is 6BV / h, and 5 times the resin volume is eluted ...

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Abstract

The invention relates to a method to extract high-purity salidroside from ligustrum lucidum which is dry mature fruit of oleaceae ligustrum, belonging to the field of pharmacy of Chinese drugs. The method takes ethanol at certain concentration to distil material through reflux; the extract solution is filtrated, condensed and dried; the extract solution is dispersed through water and then centrifugated and the supernatant fluid is further diluted; the diluted liquid runs through a AB-8 macroporous resin column and is washed with water to get rid of the impurities and then is eluted with aqueous ethanol solution at a concentration of 50%; the liquids eluted with aqueous ethanol solution at a concentradtion of 50% are combined; the combined liquid is condensed and the condensate is processed with silica gel column chromatography and then is recrystalized, finally obtaining high-purity salidroside. The salidroside prepared with the method is large in production, high in yield and purity. The materials used in the method are easy to get, the method eases the shortage of salidroside, and part of the materials and reagents can be reused, thus reducing preparation cost.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for isolating high-purity salidroside from plant Ligustrum lucidum. Background technique [0002] Salidroside, English name salidroside, chemical name "p-hydroxyphenyl alcohol-β-D-glucopyranoside". It is an active compound extracted from the medicinal plant Rhodiola rosea. Salidroside has liver protection, anti-aging, anti-fatigue and bidirectional regulation effects similar to ginseng. It is a highly promising environmental adaption meta-drug. At present, the main methods for preparing salidroside include chemical synthesis and extraction from plants of the genus Rhodiola. However, Rhodiola is mostly born on limestone, granite, mountain water or valley rocks at an altitude of 3500 to 5000 meters. Difficult to obtain and expensive. The content of salidroside in cultivated goods is low (less than 0.2%). For the preparation method of salidroside, see two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18A61K36/185
CPCY02P20/582
Inventor 石任兵周文娟田恒康刘斌
Owner 石任兵
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