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Technique for extracting and separating salidroside from glossy privet fruit

A technology for crude salidroside and salidroside, which is applied in the field of extraction and separation technology of salidroside in Oleaceae plant Ligustrum lucidum, can solve the problems of low content of crude salidroside and the like, and achieves easy subsequent processing. , the effect of alleviating insufficient resources and simple operation methods

Inactive Publication Date: 2014-05-07
魏园
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Problems solved by technology

[0003] In Chinese patent CN200510111859.0, a method for extracting salidroside from Ligustrum lucidum is described, but the content of crude salidroside prepared by this method is relatively low
So far, there is no patent related to the method of hydrolysis to increase the content of salidroside in Ligustrum lucidum

Method used

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  • Technique for extracting and separating salidroside from glossy privet fruit
  • Technique for extracting and separating salidroside from glossy privet fruit
  • Technique for extracting and separating salidroside from glossy privet fruit

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preparation example Construction

[0041](b) Preparation of reference substance solution: Accurately weigh 1.1 mg of salidroside standard substance to obtain 1.1 mg / mL salidroside standard substance solution; accurately weigh 2.25 mg of tyrosol standard substance to obtain 225 μg / mL tyrosol standard solution. Then accurately draw 0.3 mL and 0.1 mL of the above two standard solutions in turn to make a mixed solution of the two standards, in which the concentration of the standard salidroside is 330 μg / mL, and the concentration of the standard tyrosol is 22.5 μg / mL .

[0042] (c) Preparation of the test solution: Weigh the dry extract of Ligustrum lucidum water extract (or the dry extract after hydrolysis), add 20% methanol to dissolve, set the volume in a 10 mL volumetric flask, and pass through a 0.45 μm micro Pore ​​filter membrane, take the continued filtrate, that is.

[0043] (d) Determination method: Precisely draw and mix 10 μL of the reference substance solution and 20 μL of the test solution, inject i...

Embodiment 1

[0051] Take the dried and mature fruit of Ligustrum lucidum of the genus Ligustrum in the family Oleaceae, crush it to a coarse powder, add 8 times the amount of water to reflux and extract 3 times, each time is 2 hours, combine the extracts, and recover the solvent under reduced pressure at 80 ° C to obtain dried Extract concentration of 0.05 g / mL is Ligustrum lucidum concentrate, of which the yield of dry extract is 25.1%, and the content of salidroside is 1.65%. The dry extract concentration of 0.05 g / mL means that 1 mL of concentrated solution contains 0.05 g of dry extract, that is, 0.5 mg of dry extract can be obtained by recovering the solvent from 1 mL of concentrated solution to dryness.

[0052] Take 2 mL of the concentrated solution, add 100 μL of glacial acetic acid, that is, glacial acetic acid with a volume ratio of 20:1, and reflux at 100 °C for 20 hours. Adjust to neutral with sodium bicarbonate, recover the solvent under reduced pressure at 80°C to dryness...

Embodiment 2

[0064] Take the dried and mature fruit of Ligustrum lucidum, which is a plant of the genus Ligustrum in the family Oleaceae, crush it into a coarse powder, add 10 times the amount of water to reflux and extract 3 times, each time is 2 hours, combine the extracts, and recover the solvent under reduced pressure at 80 ° C to obtain Ligustrum Chaste seed concentrate.

[0065] Add glacial acetic acid with a volume ratio of 20:1 to the concentrate, and reflux at 100°C for 20 hours. Adjust to neutral with sodium bicarbonate, and recover the solvent to dryness under reduced pressure at 80°C to obtain salidroside hydrolyzate extract. Add a certain volume of distilled water to the hydrolyzate extract, so that the volume ratio of the amount of medicinal material to the dispersed solution is 1:2 (the amount of medicinal material refers to the weight of Ligustrum lucidum g: solution volume mL). Then centrifuge at a speed of 5000 rpm for 40 minutes, pour out the supernatant, which is ...

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Abstract

The invention belongs to the technical field of medicine, and relates to a technique for extracting and separating salidroside from glossy privet fruit, which comprises the following steps: (1) pulverizing glossy privet fruit as a raw material, and extracting the pulverized glossy privet fruit with water or ethanol as an extraction solvent, thereby obtaining an extracting solution; (2) concentrating the extracting solution in the step (1), and hydrolyzing by adding glacial acetic acid into the concentrated solution; and (3) preparing the hydrolysate in the step (2) into the crude salidroside product. In the invention, the salidroside is extracted and separated from the plant other than Rhodiola rosea, thereby relieving the pressure of inadequate resources; the acid hydrolysis method is utilized to enhance the content of salidroside in the glossy privet fruit; and the operational method is simple, the later processing is easy, the raw material for hydrolysis is nontoxic, the filler is cheap, and the solvent part can be recycled and reutilized, thereby saving the cost.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an extraction and separation process of salidroside in privet fruit of Oleaceae plant. Background technique [0002] Ligustrum lucidum is Oleaceae plant privet ( Ligustrum lucidum Ait.)’s dried and mature fruit is a traditional Chinese medicine commonly used to strengthen the body and strengthen the body. It has the effects of nourishing the liver and kidney, improving eyesight and black hair. Ligustrum lucidum contains chemical components such as oleanolic acid, salidroside, tyrosol and various split ring iridoid glycosides. Salidroside, English name salidroside, chemical name "p-hydroxyphenyl alcohol-β-D-glucopyranoside", has various pharmacological effects such as dilating resistance blood vessels and capacity blood vessels, anti-myocardial ischemia, anti-hepatic fibrosis, etc. , has been widely developed and applied as medicine and health food. The preparatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 王飞黄君梅陈前锋蒲文臣张国林魏园
Owner 魏园
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