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Qualitative analysis detection method for high polarity sugar-reducing chemical medicament in traditional Chinese medicine

A detection method and qualitative analysis technology, applied in the direction of analyzing materials, testing pharmaceutical preparations, measuring devices, etc., can solve problems such as long separation time, uninvolved, and complexity, and achieve the effects of wide coverage, strong specificity, and high sensitivity

Inactive Publication Date: 2011-06-08
北京市东城区药品检验所
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Problems solved by technology

[0010] However, none of the above-mentioned literatures involves the detection of α-glucosidase inhibitor drugs acarbose and voglibose; Drugs" (Chinese Pharmacist, Volume 8, No. 10, 2005, Page 827-829) Determination of possible 3 in Chinese medicine hypoglycemic preparations using reversed-phase high-performance liquid chromatography-diode array detection (RP-HPLC-DAD) Chemicals-phenformin, acarbose, glibenclamide, chromatographic conditions: Agilent ZORB-AX ODS C18 (250mm×4.6mm, 5μm); mobile phase: acetate buffer (10mmol / L acetic acid Ammonium, 20mmol / L triethylamine, adjust pH5.5 with acetic acid) and acetonitrile, gradient elution, flow rate: 0.6mL min -1 ;Detection wavelength: 230nm; However, this process requires gradient elution, which is a bit complicated, and the separation time is long, which is not conducive to rapid identification

Method used

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  • Qualitative analysis detection method for high polarity sugar-reducing chemical medicament in traditional Chinese medicine
  • Qualitative analysis detection method for high polarity sugar-reducing chemical medicament in traditional Chinese medicine
  • Qualitative analysis detection method for high polarity sugar-reducing chemical medicament in traditional Chinese medicine

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Experimental program
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Effect test

Embodiment 1

[0045] 1. High performance liquid chromatography conditions

[0046] The mobile phase is a phosphate-acetonitrile mobile phase system; the mobile phase system is composed of A and B, A is 600 mg potassium dihydrogen phosphate, 279 mg disodium hydrogen phosphate is dissolved in 1 liter of pure water, B is acetonitrile, and the volume ratio is A:B was 30:70, and the flow rate of the mobile phase was 1.0ml / min. The chromatographic column is a Hypersil amino chromatographic column, the column temperature is 30° C., and the specification is 5 μm in particle size, 4.6 mm×250 mm. The detection wavelength is 195nm. The injection volume was 20 μl.

[0047] 2. Analysis of the reference solution

[0048] Mix highly polar hypoglycemic drugs together to prepare a methanol solution, in which the concentration of acarbose is 1000 μg / ml, the concentration of phenformin hydrochloride is 5 μg / ml, the concentration of metformin hydrochloride is 10 μg / ml, and the concentration of voglibose Co...

Embodiment 2

[0054] In the high performance liquid chromatography conditions, the flow rate of the mobile phase is 0.8ml / min, the column temperature is 20° C., and the rest are the same as in Example 1.

Embodiment 3

[0056] In the high performance liquid chromatography conditions, the flow rate of the mobile phase is 1.2ml / min, the column temperature is 45° C., and the rest are the same as in Example 1.

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Abstract

The invention discloses a qualitative analysis detection method for illegally mixed high-polar chemical anti-diabetic components in anti-diabetic traditional Chinese medicine products. In the method, a moving phase is a phosphate-acetonitrile moving phase system; the flow rate is between 0.8 and 1.2ml / min; a chromatographic column adopts a Hypersil amino chromatographic column, the column temperature is between 20 and 45 DEG C, metformin hydrochloride, phenformin hydrochloride, acarbose and voglibose can realize the complete separation. When retention time of a chromatographic peak in an anti-diabetic traditional Chinese medicine product is consistent with that of anti-diabetic medicine and the apparent absorption is shown out, which indicate that the anti-diabetic medicine is contained in the sample to be tested. The method has the advantages of quickness, simplicity, convenience, high sensitivity, strong specialization, broad coverage and so on.

Description

technical field [0001] The invention provides a qualitative analysis and detection method for illegally mixing high-polarity hypoglycemic chemical drugs in hypoglycemic traditional Chinese medicine products, which belongs to the drug quality detection system. Background technique [0002] Diabetes has become a worldwide health problem, and the prevalence of diabetes in my country is also increasing year by year. The new patients are mainly type 2 diabetes, and oral hypoglycemic drugs play an important role in the treatment of such patients. In recent years, biguanides and α-glucosidase inhibitors are commonly used clinical hypoglycemic drugs. Some traditional Chinese medicine hypoglycemic preparations have also appeared in the folk. Folk Chinese medicine preparations are pure Chinese medicine preparations with few side effects and can be taken in large quantities. Clinically, patients who take these Chinese medicine hypoglycemic drugs are poisoned. Therefore, it is necessar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N33/15
Inventor 杨秉呼赵秀梅朝克图刘小辉郭栋王江华仵淑红聂然孙建慧马家华
Owner 北京市东城区药品检验所
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