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Construction method of mice infection model of Suis Eperythrozoonsis

A porcine eperythrozoon and porcine eperythrocytic technology, applied in the field of bioengineering, can solve the problems of affecting test results, one-sidedness, and not a single source of infection

Inactive Publication Date: 2008-10-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can also establish the animal model of porcine Eperythrozoon, there are two defects in this model: (1) the use of whole blood to attack the virus will inevitably bring in impurities such as white blood cells, and the source of infection is not single, which will affect test results
(2) Modeling with mice in an immunosuppressed state cannot truly reflect the state of natural disease, which is relatively one-sided

Method used

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  • Construction method of mice infection model of Suis Eperythrozoonsis
  • Construction method of mice infection model of Suis Eperythrozoonsis

Examples

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Effect test

Embodiment 1

[0022] Example 1 Construction of Eperythrozoon porcine SPF Kunming mouse infection model

[0023] (1) Separation and purification of porcine Eperythrozoon

[0024] First, 200 ml of pig blood suffering from porcine eperythrozoonosis was aseptically collected, centrifuged at 2000 r / min for 10 min, and the supernatant was removed. Add the precipitated erythrocyte sludge to an equal volume of normal saline to resuspend the erythrocyte suspension. Add an equal volume of lymphocyte separation medium and red blood cell suspension into a new centrifuge tube, centrifuge at 2500r / min for 20min, remove the uppermost and middle white blood cell layers, and remove the lower red blood cell layer. Resuspend the erythrocyte layer with an equal volume of normal saline, and after bathing in 50°C water for 15 minutes, the separation of Eperythrozoon and erythrocytes can be observed under an oil microscope. At this time, centrifuge at 2000r / min for 10min, and take the supernatant into a centrifu...

Embodiment 2

[0039] Example 2 Construction of Eperythrozoon porcine SPF BALB / c mouse infection model

[0040] (1) Separation and purification of porcine Eperythrozoon

[0041] First, 200 ml of pig blood suffering from porcine eperythrozoonosis was aseptically collected, centrifuged at 2000 r / min for 10 min, and the supernatant was removed. Add the precipitated erythrocyte sludge to an equal volume of normal saline to resuspend the erythrocyte suspension. Add an equal volume of lymphocyte separation medium and red blood cell suspension into a new centrifuge tube, centrifuge at 2500r / min for 20min, remove the uppermost and middle white blood cell layers, and remove the lower red blood cell layer. Resuspend the erythrocyte layer with an equal volume of normal saline, and after bathing in 50°C water for 15 minutes, the separation of Eperythrozoon and erythrocytes can be observed under an oil microscope. At this time, centrifuge at 2000r / min for 10min, and take the supernatant into a centrifug...

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Abstract

The invention provides a construction method of the SPF mouse infection model of an epperythrozoon in the biological technology field, which includes the steps of (1) the separation and purification of the epperythrozoon, (2) the feeding of the SPF mouse in the isolation chamber for the fumigation and the disinfection, (3) the vaccination, (4) the detection evaluation of the animal model. The invention successively constructs the SPF mouse infection model of the epperythrozoon and is verified by three methods, including fresh blood squash detection, PCR detection and transmission electron microscope detection. The model can be applied to the deep researches in the aspects of the etiology, the pathogenic force, the pathology and the immunology of eperythrozoonosis, etc.

Description

technical field [0001] The invention relates to a method for constructing an animal model in the technical field of bioengineering, in particular to a method for constructing a mouse infection model of porcine Eperythrozoon. Background technique [0002] Eperythrozoonosis is caused by Eperythrozoon parasitizing on the surface of red blood cells, plasma and bone marrow, and its main clinical features are decreased hematocrit, decreased hemoglobin concentration, leukocytosis, anemia, jaundice and fever. of zoonotic diseases. The disease has a wide range of distribution and many infected hosts, causing great harm to human health and the development of animal husbandry. In recent years, medical workers at home and abroad have conducted in-depth research on Eperythrozoonosis. Especially since the 1980s, the outbreak of Eperythrozoonosis in humans, livestock and poultry has been reported, which has attracted more and more attention from domestic and foreign scholars. The disease...

Claims

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Application Information

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IPC IPC(8): A61K49/00C12N5/06G01N33/50G01N13/10C12Q1/68
Inventor 崔立华修国余飞杨志彪袁聪利
Owner SHANGHAI JIAO TONG UNIV
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