Epitope screening method capable of exciting anti-mycobacterium tuberculosis protective immunological reaction of body and uses

A technology of protective immunity and antigenic epitopes, applied in the field of molecular immunology, can solve problems such as delayed treatment timing, unstable immune protection effect, disseminated tuberculosis, etc., to help prevent and treat, and promote multi-drug resistance The Effects of Sexual Difficulties

Active Publication Date: 2008-10-22
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

With the continuous emergence of drug-resistant strains of Mycobacterium tuberculosis in recent years and BCG as the only vaccine currently used to prevent tuberculosis, the immune protection effect is unstable; after vaccinatio

Method used

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  • Epitope screening method capable of exciting anti-mycobacterium tuberculosis protective immunological reaction of body and uses
  • Epitope screening method capable of exciting anti-mycobacterium tuberculosis protective immunological reaction of body and uses
  • Epitope screening method capable of exciting anti-mycobacterium tuberculosis protective immunological reaction of body and uses

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Experimental program
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Effect test

Embodiment 1

[0026] 1 Materials and methods

[0027] 1.1 Tuberculin was positive in 4 cases of rhesus monkeys from wild sources, all of which were confirmed tuberculosis by autopsy. One tuberculosis-negative healthy monkey injected with BCG was used as a positive control, and one tuberculosis-negative healthy monkey without BCG injection was used as a negative control.

[0028] 1.2 Reagents and kits:

[0029] Lymphocyte separation medium ACK, PRMI1640 medium and fetal bovine serum were purchased from Dalian Bao Biological Company;

[0030] Peptides were synthesized by Biotech Biotech.

[0031] HRP-labeled goat anti-monkey IgG (H+L) antibody was purchased from Jingmei Company.

[0032] 1.3 Main instruments

[0033] Type 550 microplate reader was purchased from Bio-Rad;

[0034] 1.4 Antigen epitope prediction and molecular mimic peptide synthesis

[0035] Such as figure 1, epitope prediction was performed according to the following procedure. According to the retrieved literature, we...

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Abstract

The invention relates to a selection method for epitope which can stimulate the protective immune response of the body anti-mycobacterium tuberculosis and the function thereof, in particular to the molecule mimic peptide of the epitope with vaccine development prospect from the mycobacterium tuberculosis and the coding DNA thereof. The selection method of epitope which can stimulate the protective immune response of the body anti-mycobacterium tuberculosis and the function thereof provides T lymphocyte epitope contained in one important gene Ag85B in the research of mycobacterium tuberculosis and the method of deducting or selecting the epitope, which is beneficial to further developing novel multivalent and poly epitope tuberculosis vaccine and prevent and control the happening and development of tuberculosis; the method makes a foundation of the future development of synthesizing peptide vaccine epitope vaccine and dna vaccine by using epitope and provides molecule mimic peptide of epitope which can stimulate the protective immune response of the body anti-mycobacterium tuberculosis and the peptide has the amino acid sequence of FVRSSNLKFQDAYNA(SEQ ID NO:1).

Description

technical field [0001] The invention belongs to the field of molecular immunology, and specifically relates to an antigenic epitope screening method capable of stimulating a body's protective immune response against Mycobacterium tuberculosis (MTb) and its application. Background technique [0002] Tuberculosis is a chronic infectious disease. It is a necrotic disease caused by a bacterium called Mycobacterium tuberculosis (Mtb) invading the body. It may occur in any part or organ of the body, among which the lungs are the most vulnerable. At present, Mycobacterium tuberculosis is still an important pathogenic microorganism that seriously endangers human health. Every year, it will cause infection of more than 10 million people and cause death of about 3 million people worldwide. With the continuous emergence of drug-resistant strains of Mycobacterium tuberculosis in recent years and BCG as the only vaccine currently used to prevent tuberculosis, the immune protection effect...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/00C12N15/09G01N33/68A61K39/00A61P31/06
Inventor 代解杰罕园园孙晓梅高家红唐东红匡德宣
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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