Biological magnification method of gamma-propalanine(GABA) in rice polishings

Abstract

The invention relates to a biological enrichment method of gamma-aminobutyric acid (GABA) from rice bran, in particular to a method for enriching and producing gamma-aminobutyric acid (GABA) from rice bran by enzyme biotechnology, which belongs to the field of food biotechnology. The method comprises the following steps: selecting rice bran as the raw material, extracting GAD in rice bran by phosphate buffer solution to obtain crude enzyme liquid, preparing fixed GAD from the crude enzyme liquid by using biomaterial chitosan as carrier and glutaraldehyde as crosslinking agent, adding a substrate glutamic acid or sodium glutamate to prepare GABA reaction liquid, centrifuging to obtain supernate, adding ethanol into the supernate to remove polysaccharide impurity, ultra-filtering to obtain high-concentration gamma-aminobutyric acid solution, or further concentrating and drying to obtain powdery product. The biological enrichment of GABA in rice bran can be used for producing edible GABA, and the GABA can be used as additives for functional health food, thus greatly improving added value of rice bran and achieving higher economic and social benefits.

Description

A bioaccumulation method of gamma-aminobutyric acid (GABA) in rice bran technical field The invention discloses a bio-enrichment method for gamma-aminobutyric acid (GABA) in rice bran, which relates to a method for enriching and producing gamma-aminobutyric acid by using rice bran as a raw material, and belongs to the field of food biotechnology. Background technique Rice is the largest grain variety in my country, with an annual production of about 185 million tons, accounting for 42% of the country's total grain output. Rice bran is a by-product of rice processing. Rice bran accounts for 5-8% of the weight of rice. The annual output in my country is about 10 million tons. It is a renewable resource with a wide range of quantities. The United Nations Industrial Development Organization calls rice bran an underutilized raw material. Modern research proves that rice bran is the essence of rice grain, which concentrates 64% of the nutrients. At present, foreign countries h...

AI Technical Summary

Problems solved by technology

There is a method for preparing high-concentration γ-aminobutyric acid powder from rice bran, but the separation and purification of GABA is still difficult