The invention discloses a preparation method of codonopsis pilosula sections, which comprises the following steps: cleaning fresh codonopsis pilosula medicinal materials to remove impurities, cleaning, cutting the fresh codonopsis pilosula into sections, cleaning again, drying in a drying oven until the water content is lower than 10%, pulverizing the dried codonopsis pilosula, and drying again until the water content is lower than 5%, and obtaining codonopsis pilosula powder; and then performing supercritical extraction treatment on the codonopsis pilosula powder. A supercritical extraction technology is adopted for extracting the active ingredients of codonopsis pilosula, the extraction rate is remarkably superior to that of a traditional extraction technology, and the supercritical extraction technology has the advantages that extraction and separation can be completed at a time, the extraction efficiency is high, the active ingredients are well reserved, and no solvent is left. According to the supercritical extraction process disclosed by the invention, the components of an entrainer are optimized, so that the extraction rates of saccharides and glycosides are stable, and meanwhile, the extraction of alkaloid can be reduced.