RT-PCR method for testing hantavirus genome

An RT-PCR, Hantavirus technology, applied in the field of Hantavirus genome detection, to achieve the effect of high positive detection rate, increased genus specificity, and high detection rate
CN101294226BInactive Publication Date: 2010-09-01WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2010-09-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting hantaan virus strain genome. The method has the following steps: (1) two pairs of hantaan virus general primers are designed; (2) the hantaan virus detection method comprises the following steps that: first, viral specific RNA extraction kit is utilized to extract the total RNA in a serum sample; second, a nucleic acid analyzer is adopted to detect the RNA content, and the RNA is taken to perform the RT-PCR reaction; third, RT-PCR:RT uses HV group-specific primers such as P0, PCR uses HV general primers such as P1P2 and P3P4; fourth, viral nucleic acid amplified results are observed through agarose gel electrophoresis, and a specific nucleic acid band appears. The method is intuitionistic and predominant, the sensibility is good, and the effects are good; the serum detection rate in recent ten years is 79.2 percent, and the serum detection rate in twenty years ago can still reach to 70.5 percent; the method is applied to the laboratory detection of hantaan virus infection and the molecular epidemiological survey.
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Description

technical field

[0001] The present invention relates to the field of virus detection, in particular to a method for detecting the hantavirus (HV) genome, comprising designing two pairs of general primers for the novel hantavirus, studying experimental conditions, and establishing a RT-PCR method for the virus genome, Serum samples of Hantavirus in different ages were tested. Background technique

[0002] Viral diseases are a class of infectious diseases that seriously threaten human health. More than 85% of infectious diseases are caused by viruses, and important viruses endanger national security and social development. Hantaviruses (HV) are the pathogens of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). It is listed by the World Health Organization as a biological weapon that can be transmitted through aerosols. The main features of HFRS / HPS are fever, hemorrhagic renal impairment, and respiratory failure. my country is the country...

Claims

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