Sheet stainer

Abstract

The invention relates to a section staining machine, which comprises a machine body; a reaction cylinder, a wax cylinder, a reagent glass and a waste liquid cylinder positioned on the machine body; a reagent delivering device for delivering the reagent in the reagent glass to the reaction cylinder; and a waste liquid delivering device for delivering the waste liquid from the reaction cylinder to the waste liquid cylinder. The reaction cylinder is connected with the wax cylinder through a pipeline; a control valve is arranged on the pipeline; the reaction cylinder is connected with the reagent glass through the reagent delivering device; and the waste liquid cylinder is connected with the reaction cylinder through the waste liquid delivering device. In the section staining machine, the wax cylinder is connected with the reaction cylinder through the pipeline, and a reagent delivering pipeline is used for delivering reagents used for dehydrating, dewaxing, staining and cleaning to the reaction cylinder, so that a biological sample in the reaction cylinder can be dehydrated, dewaxed and stained, the dewaxed biological sample can be sectioned and washed with a washing reagent in the reaction cylinder, and the waste liquid in the reaction cylinder after cleaning can be delivered to the waste liquid cylinder through a waste liquid delivering pipeline, thus avoiding cross contamination of the reagents used for dehydrating, dewaxing and staining in the process of preparing the biological sample section, sectioning and staining, preventing the damage to human bodies due to reagent volatilization, and meanwhile ensuring the dehydrating and waxing process and the sectioning and staining process of the biological sample are completed in the same reaction container.

Description

technical field [0001] The invention relates to a device for preparing and staining medical or biological specimens. Background technique [0002] The production of medical or biological specimens generally includes the tissue preparation process and the staining process. The tissue preparation process is generally carried out in the following order: tissue washing, tissue dehydration with a dehydrating agent such as ethanol, and tissue transparency with a transparent agent such as xylene , tissue soaking in wax, tissue embedding, and finally take out the slices to get the specimen sheet. In the prior art, the tissue slice process is generally completed in a dehydrator. The principle is to dehydrate the biological tissue through the action of gradient ethanol and xylene, and then Soak it in melted paraffin to obtain tissue wax blocks, take out the tissue wax blocks and slice them with a microtome; the staining process includes the processes of dewaxing, staining, dehydration...

AI Technical Summary

Problems solved by technology

[0002] The production of medical or biological specimens generally includes the tissue preparation process and the staining process. The tissue preparation process is generally carried out in the following order: tissue washing, tissue dehydration with a dehydrating agent such as ethanol, and tissue transparency with a transparent agent such as xylene , tissue soaking in wax, tissue embedding, and finally take out the slices to get the specimen sheet. In the prior art, the tissue slice process is generally completed in a dehydrator. The principle is to dehydrate the biological tissue through the action of gradient ethanol and xylene, and then Soak it in melted paraffin to obtain tissue wax blocks, take out the tissue wax blocks and slice them with a microtome; the staining process includes the processes of dewaxing, staining, dehydration, transparency, and sealing of specimen slices. The dyeing process in the technology is generally completed by a dyeing machine. Its working principle is to use a mechanical arm to lift a basket containing specimen slices, and put them one by one into an open reagent container containing chemical reagents for a series of chemical and physical tests. Reaction to achieve the purpose of staining, the production process of the above two specimens generally has the following technical defects: 1. In the tissue production process, because alcohols such as reagent ethanol are flammable and explosive materials, xylene is poisonous materials, and Paraffin wax at room temperature is solid. During the process of washing after wax dipping, the residual solid wax after the liquid wax flows through the liquid circulation pipeline often blocks the liquid circulation pipeline and causes the reagents used to overflow and pollute the environment; 2. During the dyeing process, Reagents such as hydrochloric acid, xylene, alcohols and other reagents used are strong corrosive chemicals and have strong volatility. The material for making the manipulator is steel, which is corroded by strong corrosive hydrochloric acid and xylene. , its working life is low, and it may fail during the operation and cause the specimen to be scrapped; the staining process is a process in which the reagent reacts chemically and physically on the specimen, which is dependent on time and temperature, but due to the strong corrosion of the reagent used As a result, containers for containing reagents can only be made of engineering plastics, and the characteristics of engineering plastics happen to have good heat insulation and low heat resistance, so this technology can only work under the external temperature environment, so in In practical applications, the same dyeing reaction will often adjust the program due to external temperature changes, making the dyeing work complicated; When the basket with specimen slices is moved between open containers containing reagents, the reagents left on the basket will cause cross-contamination between reagents, so the loss of reagents is large, and due to the volatility of reagents, most of the work The environment is a mixture of man and machine, which to a large extent has caused harm to human health, and long-term damage can also cause cancer of human tissues

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