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Device and method for fast detecting PSA prostate specific antigen

A prostate-specific and rapid determination technology, which is applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, to achieve good accuracy, high measurement precision, and low cost

Inactive Publication Date: 2008-10-29
NINGBO UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The primary technical problem to be solved by the present invention is to provide a device for rapid determination of PSA prostate-specific antigen with high measurement accuracy, no radioactive pollution, simple operation, less interference, and easy industrialization

Method used

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  • Device and method for fast detecting PSA prostate specific antigen
  • Device and method for fast detecting PSA prostate specific antigen
  • Device and method for fast detecting PSA prostate specific antigen

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Experimental program
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Embodiment

[0051] Reagents and Instruments

[0052] Mercaptobutylene diamine is a product of Sigma Corporation in the United States. Hydrogen peroxide (30%) (H 2 o 2 ) was purchased from Shanghai Biochemical Reagent Company. AuCl 3 HCl.4H 2 O (Au%>48%) and all other reagents were of analytical grade and used directly without purification. PSA ELISA kit and radioimmunoassay kit, including 96-well plate (DPC company, USA) immobilized with PSA monoclonal antibody in advance. The ELISA kit includes a series of anti-PSA standard solutions with different mass concentrations (0-500ng / mL). CNBr active sepharose 6MB microbeads were purchased from (Emerson Corporation, USA). All other reagents were of analytical grade, and the water used in the experiment was secondary water. Eluent: pH 6.2 0.5mol / L NaCl+0.1mol / L PBS.

[0053] A three-electrode system was used in the experiment. Gold disc electrode (φ=0.5mm) and platinum electrode were used as working electrode and counter electrode respec...

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Abstract

The invention relates to a device and method used for fast measuring PSA prostate specific antigen, and is characterized in that the invention comprises the following steps: the signal output of an electrochemical reaction tank (1) a working electrode of which is coated with Rul and an anti-PSA thin film is connected with the signal input of a voltammeter (2); the signal output of the voltammeter (2) is connected with the signal input of a signal collecting device (3); the output of the signal collecting device (3) is connected with the signal input of a signal processing device (4); the invention also discloses the method, has simple equipment, good selectivity and accuracy, easy realization of on-line detection and mass detection, high measuring precision which can realize the detection of trace amount PSA in blood serum, no problem of radiation protection and pollution prevention, wider measuring range and low cost.

Description

technical field [0001] The invention relates to a device and method for measuring PSA prostate specific antigen, especially a device and method for measuring PSA prostate specific antigen in serum. Background technique [0002] PSA prostate specific antigen is the best marker of prostate disease. When it is greater than 10ng / ml, the sensitivity of prostate cancer is 99%, and it can also increase for prostatic hypertrophy and acute prostatitis. Therefore, establishing a simple and sensitive method has important diagnostic significance for the determination of PSA in serum. The methods commonly used to detect PSA in serum are limited due to the small amount of PSA. [0003] Currently, enzyme-linked immunosorbent assay (ELISA) and chromatographic techniques are used to detect sample-treated PSA in serum samples. However, the analysis and determination of PSA by chromatographic technology has the following disadvantages: time-consuming and long pretreatment time. In recent ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/416
Inventor 干宁侯林熙李天华徐伟民陈妮
Owner NINGBO UNIV
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