Medicine uses of herpetone
A technology for the use of polone, which is applied in the field of chemical drugs and can solve problems such as reports on the medical use of bolengone that have not yet been seen.
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Embodiment 1
[0009] The preparation of embodiment 1 polengone
[0010] Take 10 kg of dried and pulverized samples of pollen melon seeds, degrease with petroleum ether, and then heat and reflux extraction with 95% ethanol in a water bath (3 × 10 L), each time for 2 hours, distill the ethanol off under reduced pressure to obtain about 614 g of liquid extract. Add appropriate amount of quartz sand to the liquid extract and mix well, then place it in a Soxhlet extractor, heat and extract it with petroleum ether (boiling range 60-90°C), chloroform, ethyl acetate, and 95% ethanol in sequence, and depressurize the extract After recovery, 27g of chloroform extract, 32g of ethyl acetate extract and 195g of ethanol extract were obtained.
[0011] 20 g of the ethyl acetate extract was subjected to silica gel column chromatography with chloroform-methanol gradient elution. The fraction eluted with chloroform-methanol (99:1) was subjected to silica gel column chromatography, and the fraction eluted wi...
Embodiment 2
[0015] Example 2 The protective effect of polengone on DMSO-injured rat liver cells
[0016] Hepatic parenchymal cells were obtained by in vitro culture. Disperse the hepatic parenchymal cells with DMEM, and the cell count should not be less than 2×10 5 cells / ml, the cell viability was greater than 90%. Add the dispersed cells to the 96-well plate, 80 μl per empty.
[0017] In the experiment, 3 groups were set up, except for the blank control group, including the modeling group and the drug administration group, adding DMSO with a final concentration of 8mmol / L to each space to create a liver cell injury model; / ml, and incubated in a carbon dioxide incubator for 24 hours.
[0018] Incubate cells at 37°C, 5% CO 2 After culturing in the incubator for 24 hours, add 50 μl of MTT (0.25 mg / ml), 37°C, 5% CO 2 The incubator was cultured for 4 hours, the culture solution was aspirated, and then 150 μl of DMSO was added to stop the reaction, and the OD value was measured with a mi...
Embodiment 3
[0023] Embodiment 3 polengone is to CCl 4 Protective effect of injured rat liver cells
[0024] Hepatic parenchymal cells were obtained by in vitro culture. Disperse the hepatic parenchymal cells with DMEM, and the cell count should not be less than 2×10 5 cells / ml, the cell viability was greater than 90%. Add the dispersed cells to the 96-well plate, 80 μl per empty.
[0025] A total of 6 groups were set up in the experiment. In addition to the blank control group, including the modeling group and the drug administration group, CCl with a final concentration of 8mmol / L was added to each blank. 4 , to create a liver cell injury model; the administration group was added with polengone at a concentration of 0.5mg / ml, 0.25mg / ml, 0.125mg / ml, and 0.0625mg / ml, and placed in a carbon dioxide incubator for incubation for 24 hours.
[0026] Incubate cells at 37°C, 5% CO 2 After culturing in the incubator for 24 hours, add 50 μl of MTT (0.25 mg / ml), 37°C, 5% CO 2 The incubator was...
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