Reflection type substrate

Abstract

The invention discloses a reflection-typed substrate which is a specular reflection-typed substrate for a biochip applied to the biological field, comprising a base and a chemical modification layer supporting and combining with biological molecules on the base; a film layer for reducing the diffuse scattering of a fluorescence signal is arranged between the base and the chemical modification layer supporting and combining with the biological molecules. In a condition that the chemical modification layer supporting and combining with the biological molecules of the reflection-typed substrate ensures combination capability between the substrate and the biological molecules (the combination capability is enhanced by the chemical modification layer to a certain extent), the arrangement of the film layer for reducing the diffuse scattering of the fluorescence signal further promotes the strength and flexibility of the signal when the biochip is used for detection, solves the situation that on a solid medium, the fluorescence signal scatters in a diffuse way towards each direction, causes the fluorescence signal of the marked biological molecules to reflect directionally and a biological signal with high contrast ratio, high signal strength and high flexibility to be obtained at a signal collection end (a photoelectric magnification tube (PMT) and matrix of charge coupled device (CCD)) of scanning equipment.

Description

Technical field [0001] The invention relates to a coated mirror reflection type substrate for a biological chip in the biological field. Background technique [0002] The Human Genome Project (HGP), launched in 1990, launched the greatest scientific research program for human beings to understand themselves. HGP published accurate and high-quality human genome sequencing results in June 2001, and published high-quality Accurate and detailed annotated results of the Human Genome Study, which marks the completion of the Human Genome Project ahead of schedule. At the same time, significant progress has been made in the sequencing of the genomes of various species on Earth. [0003] With the publication of the whole genome sequence of organisms, the focus of research has shifted to the era of post-genome projects aimed at clarifying the structure and function of all genes in various species, and using them to benefit the organism itself, which includes post-genome projects, Cl...

AI Technical Summary

Problems solved by technology

[0013] Existing biochip substrates, in the process of developing and manufacturing biochips, either cannot guarantee the good combination of biomolecules and substrates, or it is difficult to intuitively obtain complete fluorescent signals of biomolecular markers during detection, that is, biochip scanning detection The quality is relatively poor, and it needs to rely heavily on computer software reprocessing based on complex mathematical models in the later stage. There are various types of signal distortion and distortion problems, which affect the judgment and reading of biological information.

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