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Chemiluminescent detection system

A measurement device, chemiluminescence technology, applied in measurement devices, chemiluminescence/bioluminescence, chemical instruments and methods, etc., can solve the problems of inability to supply, change in luminescence intensity, sequence determination or DNA detection accuracy, and achieve analytical production. The effect of improving the quantity and measuring sensitivity

Inactive Publication Date: 2008-12-24
HITACHI LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At this time, if the reaction product PPi or ATP produced in each micro-reaction tank is mixed into the adjacent reaction tank (crosstalk occurs), there is a problem that the accuracy of sequence measurement or DNA detection will decrease.
Specifically, PPi generated during DNA extension diffuses into adjacent micro-reaction chambers, causing luminescence as if an extension reaction occurs in the adjacent micro-reaction chambers, and there is a problem that the intensity of luminescence changes.
[0016] On the other hand, in order to suppress the diffusion of the products produced by the extension reaction, membranes or packing beads can also be used, but in this case, the substances necessary for DNA extension or chemiluminescence cannot be quickly supplied to the inside of the micro-reaction tank. , and cannot exclude the remaining reaction substrates, that is, there is a problem that the important DNA complementary strand synthesis reaction cannot be uniformly performed in improving the accuracy of DNA analysis

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0073] figure 1 It is a diagram showing a configuration example of the chemiluminescence measurement device of the present invention. In the chemiluminescence measurement device of this embodiment, various reagents are poured into the flow cell 101 formed by the plate 201 provided with a plurality of micro reaction tanks and the transparent substrate 105, by measuring Chemiluminescence in the reaction tank 103 to determine the base sequence. In the present invention, when measuring chemiluminescence and when flowing reagents to supply reagents to the micro-reaction tanks, the thickness of the reagent flow path is changed to change the conductance, cross-sectional shape, and cross-sectional area of ​​the flow path. Accordingly, when measuring chemiluminescence, the luminous density is increased while suppressing crosstalk with adjacent micro-reaction tanks, thereby improving the measurement sensitivity of luminescence. In addition, when supplying reagents to the micro-reactio...

Embodiment 2

[0092] In Example 1, the thickness of the flow path was changed by elastically deforming the spacer, thereby suppressing the diffusion of the product from the micro-reaction tank. The same effect as in Example 1 was obtained by changing the thickness of the flow path immediately above the micro reaction tank.

[0093] Figure 8 A schematic cross-sectional view showing another example of the flow cell. The flow cell of this example is a flow cell in which stress can be applied to the transparent substrate 701 to bend it, thereby reducing the thickness of the flow path directly above the micro reaction tank 103 . Figure 8 (a) is a diagram showing a state where no stress is applied, Figure 8 (b) is a diagram showing a state where stress is applied and the thickness of the flow path is reduced. In the present embodiment, by thinning a part of the transparent substrate 701 serving as the upper plate, it has a shape that is easily deformed when stress is applied by the rod 203 ...

Embodiment 3

[0100] In this embodiment, instead of moving and deforming the transparent substrate and plate, other transparent substrates are placed in the flow path of the flow cell to suppress the diffusion of the product from the micro reaction tank. Figure 11 A system configuration example of a chemiluminescence measurement device is shown. Set electromagnet 1001 to replace figure 1 The rod 203, the driving part 1002 is provided to support and drive the electromagnet instead of the driving part 102.

[0101] Figure 12 (a) A schematic cross-sectional view showing a state in which the thickness of the flow channel of the flow cell of this example is thickened, Figure 12 (b) shows a schematic cross-sectional view in a state where the channel thickness is reduced. A transparent substrate 1101 made of polypropylene is installed in the flow path, and the surface of a neodymium magnet (main components: neodymium, iron, boron) 1102 is covered with polypropylene with a thickness of about ...

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Abstract

The present invention aims to achieve both rapid supply of reagent substances to micro-chambers and inhibition of contamination from adjacent chambers. For achieving the above objects, the shape of a flow channel in a flow cell (101) including a plate (201) having micro reaction chambers (103) is varied between the time of substance supply and the time of luminescence reaction.

Description

technical field [0001] The present invention relates to a chemiluminescence measurement device. More specifically, the present invention relates to a chemiluminescence measurement device enabling gene sequence analysis, gene polymorphism analysis, gene mutation analysis, gene expression analysis, and the like. Background technique [0002] Methods using gel electrophoresis and fluorescence detection are widely used in the determination of DNA base sequences. In this method, first, a plurality of copies of a DNA fragment to be sequence analyzed are produced. Starting from the 5' end of DNA, fluorescently labeled fragments of various lengths are produced. In addition, fluorescent labels having different fluorescence wavelengths are added in advance according to the base types at the 3' ends of these DNA fragments. By gel electrophoresis, the difference in length is identified as a difference of 1 base, and the light emitted by each fragment group is detected. The DNA termin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76C12Q1/68
CPCB01J2219/00313B01J2219/00337B01J2219/0036B01J2219/00704B01L3/5025B01L3/50273B01L3/502738B01L3/50853B01L2200/0642B01L2300/041B01L2300/046B01L2300/0654B01L2300/0819B01L2300/0877B01L2300/1822B01L2300/1827G01N21/05G01N21/76G01N2021/0325G01N2021/036G01N2021/0346
Inventor 白井正敬梶山智晴神原秀记
Owner HITACHI LTD
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