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Klebsiella pneumoniae and use thereof

A Klebsiella pneumoniae technology, applied to Klebsiella pneumoniae and its application fields in Fe reduction and humic acid reduction, to achieve the effect of strengthening the original and humic acid reduction capabilities

Active Publication Date: 2009-01-28
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many reported strains capable of reducing Fe(III) and humic acid at the same time, and Klebsiella pneumoniae has not been found to be able to reduce Fe(III) and humic acid anaerobically.

Method used

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  • Klebsiella pneumoniae and use thereof
  • Klebsiella pneumoniae and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Enrichment, separation and purification of Klebsiella pneumoniae

[0024] Pipette 5mL or weigh 5g ancient forest soil sample to inoculate the liquid separation medium; fill the culture medium with high-purity mixed gas (N 2 / CO 2 =80 / 20), the aeration time is not less than 30 minutes; after the aeration is completed, place the culture in the anaerobic workstation (N 2 / CO 2 =80 / 20) Cultivate statically at 30°C. Detect the yield of Fe(II) while observing the color change of the culture solution. When Fe(II) production continues to increase, the color of the culture fluid gradually darkens from the original orange-yellow, and finally turns to black. When the output of Fe(II) exceeds 50% of the total iron content, it is transferred to another fresh medium with an inoculum amount of 10%, and the above operation is repeated three times.

[0025] Each liter of liquid separation medium contains: 1.0g glucose, 16g ferric citrate, 2.5g NaHCO 3 , 0.25g NH 4 Cl, 0.678g NaH 2...

Embodiment 2

[0037] Example 2 Klebsiella pneumoniae MFC4 anaerobic reduction of goethite

[0038] Medium A formula: Each liter of deionized water contains 2.25g of goethite, NaHCO 3 2.5g, NH 4 Cl 0.25g, NaH 2 PO 4 ·2H 2 O 0.678g, KCl 0.1g, glucose 1.0g, vitamin solution and trace element solution each 10.0mL (vitamin solution and trace element solution composition is the same as the liquid separation medium described in Example 1), sterilized at 121 ℃ for 20 minutes, glucose Sterilize separately and mix with other ingredients after sterilization.

[0039] Preparation of bacterial suspension: Use an inoculating loop to pick a loop of activated strains in 200mL beef extract peptone liquid medium, and incubate in a shaker (30°C, 180 revolutions / min) for 16 hours to make the number of bacteria reach the exponential growth phase. The bacteria were collected by centrifugation at 8000 rpm, the supernatant was decanted, and the precipitated bacteria were suspended in 200 mL of medium A to prepare a ba...

Embodiment 3

[0044] Example 3 Klebsiella pneumoniae (K. pneumoniae MFC4) anaerobic reduction of ferrihydrite

[0045] Medium B formula: 2.5g hydrous iron ore per liter of deionized water, NaHCO 3 2.5g, NH 4 Cl 0.25g, NaH 2 PO 4 ·2H 2 O 0.678g, KCl 0.1g, glucose 1.0g, vitamin solution and trace element solution each 10.0mL (vitamin solution and trace element solution composition is the same as the liquid separation medium described in Example 1), sterilized at 121 ℃ for 20 minutes, glucose Sterilize separately and mix with other ingredients after sterilization.

[0046] Preparation of bacterial suspension: Use an inoculating loop to pick a loop of activated strains in 200mL beef extract peptone liquid medium, and incubate in a shaker (30°C, 180 revolutions / min) for 16 hours to make the number of bacteria reach the exponential growth phase. The bacteria were collected by centrifugation at 8000 rpm, the supernatant was poured, and the precipitated bacteria were suspended in 200 mL of medium B to ...

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Abstract

The invention discloses a klebsiella pneumoniae and application thereof; the invention obtains the klebsiella pneumoniae (MFC4) by separation and purification from the soil sample of an ancient forest of Zhaoqing in Guangdong, which is preserved in China culture collection and management committee general microorganisms center, with the preservation number of CGMCC2454; the invention simultaneously provides a screening method of the strain and application thereof in the reduction of Fe(III) or humic acid; the strain can reduce iron oxides with high reduction and crystallinity, such as lepidocrocite and hematite, etc.

Description

Technical field [0001] The invention belongs to the field of new strain screening technology and environmental biotechnology, and specifically relates to a strain of Klebsiella pneumoniae MFC4 and its application in Fe(III) reduction and humic acid reduction. technical background [0002] Fe(III) and humic acid are substances that are abundant in ecological environment such as sediment and soil. Fe(III) mainly exists in the form of insoluble iron oxide, and the average content in the crust is about 5.6%; humic acid is the part of humus that is soluble in alkali and insoluble in acid. It is rich in quinone groups and is a heterogeneous natural organic mixture. Under anaerobic conditions, some microorganisms can oxidize organic matter through physiological and biochemical reactions, and at the same time reduce Fe(III) oxides or humic acids, and obtain energy for growth. Such microorganisms are called Fe(III) or humic acid reducing bacteria. [0003] The reduction of Fe(III) and hum...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P9/00C12P13/00C12R1/22
Inventor 周顺桂王跃强武春媛庄莉李芳柏
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI