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Isolated liver stem cells

A technology of liver progenitor cells and stem cells, applied in the field of liver failure

Active Publication Date: 2009-01-28
UNIVERSITE CATHOLIQUE DE LOUVAIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, previous studies have used sophisticated techniques such as FACS, calcium-containing media, or specific density gradients to isolate hepatic stem cells

Method used

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Examples

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Embodiment

[0295] Liver Cell Isolation Method

[0296] Human liver cells were obtained from whole livers or liver parts from healthy cadavers or beating donors. Cells were isolated after the total blocking time (eg, 6 to 12 hours after the total blocking time), and livers were kept on ice in the University of Wisconsin medium until perfusion. Hepatocytes were isolated using a classical two-step perfusion technique {Seglen, 1976} {Stephenne, 2005}. The liver tissue was then treated with EGTA solution (Earl's Balanced Salt Solution, without Ca ++ and Mg ++ , 0.5 mM EGTA, 5 mM Hepes, 2 mg / l gentamicin and 100,000 IU / l penicillin G) and digestive enzyme solutions were perfused at 37° C. for 9 to 12 minutes each. Digestion solution (EBSS with Ca ++ and Mg ++ , 5mM Hepes, 2mg / l gentamicin and 100,000IU / I penicillin G) containing 0.9 mg / ml collagenase P and 0.03 mg / ml soybean trypsin inhibitor. The liver capsule was cut and the hepatocytes released with gentle shaking. Digestion was term...

Embodiment 2

[0349] A liver-derived progenitor or stem cell of the present invention, a cell line and / or cell population comprising said progenitor or stem cell (in particular, including but not limited to, the LMBP 6452CB line), or a progeny thereof (any (optionally genetically engineered) can be as follows.

[0350] Infuse cells by continuous injection, preferably no more than 25 to 50 x 10 6 cells / kg, preferably with an interval of 4 hours, or an interval of 8 hours, or more than 8 hours up to 1 week, or more than 1 week. Inject 250×10 for several days 6 cells / kg, or 500×10 6 cells / kg of total cells, preferably one week or preferably two weeks. Repeat the series of injections as needed, every other month, or every six months, or every other year or longer.

[0351] Surgical insertion into any vessel draining to the portal vein (preferably the inferior mesenteric vein or vein) into the portal vein by direct puncture. The catheter can be left in place for hours, preferably days, pre...

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Abstract

The present invention relates to isolated liver progenitor stem cells, and cell population thereof, wherein said progenitor stem cells originate from adult liver, esp. of human. The present invention also relates to the use of said isolated progenitor stem cells in medicine, hepatology, inborn errors of liver metabolism, transplantation, infectious diseases, liver failure. The present invention also relates to methods of isolating these cells, their culture, characterization before and after differentiation, and their use for transplantation, animal models of human disease, toxicology and pharmacology.

Description

technical field [0001] The present invention relates to isolated hepatic progenitor or stem cells from the adult liver and their use in medicine, hepatology, birth defects of liver metabolism, transplantation, infectious diseases, liver failure. The invention also relates to methods of isolating these cells, their culture, characterization before and after differentiation, and their use in transplantation, animal models of human disease, artificial organ devices, toxicology and pharmacology. Background technique [0002] The liver is a vital organ that performs many important functions such as glucose homeostasis, xenobiotic detoxification or macromolecular synthesis. Therefore, impairment of one of several liver functions can have significant health consequences. According to the World Health Organization (WHO), the global incidence of acute or chronic liver disease makes these diseases the fifth to ninth leading cause of death. So far, the only cure for advanced liver di...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 艾蒂安·索卡尔穆斯塔法·纳吉米
Owner UNIVERSITE CATHOLIQUE DE LOUVAIN
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