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Xanthan polysacchdride culture medium, preparation and application method thereof

A technology of Xanthomonas polysaccharide and culture medium, which is applied in the field of preparation of Xanthomonas polysaccharide culture medium, can solve the problems such as no public records have been found, and achieve the effects of reducing production costs, reducing pollution, and realizing technological innovation

Inactive Publication Date: 2009-02-11
金翼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the above two topics has not found any public records

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] Under normal temperature conditions, take 2 L of potato starch wastewater, filter it through a sand filter, remove solids such as potato residue, and obtain a supernatant for later use. Dilute the supernatant with tap water and adjust the COD to 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 3% of amylase according to the weight unit, stir evenly, carry out hydrolysis under the condition of 45-55 DEG C, the hydrolysis time is 72 hours, and obtain the reducing sugar solution with the concentration of 60-80g / L. Add 2% by weight of active medical stone particles (particle size: 2-10mm) to the reducing sugar solution, soak at room temperature for 8 hours to decolorize and precipitate minerals K, Ca, Na, P, Se, etc., and obtain clarification after suction filtration 2% yeast extract was added to the supernatant, and stirred evenly to obtain a Xanthomonas polysaccharide medium. Insert Xanthomonas campestris into the culture m...

Embodiment 2

[0023] Under normal temperature conditions, take 2 L of potato starch wastewater, filter it through a sand filter, remove solids such as potato residue, and obtain a supernatant for later use. Dilute the supernatant with tap water and adjust the COD to 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 0.5% of amylase by weight unit, stir evenly, carry out hydrolysis at 45-55°C for 36 hours, and obtain a reducing sugar hydrolyzate with a concentration of 60-80g / l. Add 0.5% by weight of active medical stone particles (particle size: 2-10mm) to the reducing sugar solution, soak at room temperature for 12 hours to decolorize, and precipitate minerals K, Ca, Na, P, Se, etc., after suction filtration A clear and transparent supernatant is obtained, and then 1% by weight of yeast extract is added and evenly stirred to obtain a xanthomonas polysaccharide medium. Insert Xanthomonas carotans into the culture medium, the inoculum size is 2....

Embodiment 3

[0025] Under normal temperature conditions, take 2 L of corn starch wastewater, filter it through a sand filter, remove solids, and obtain a supernatant for later use. Dilute the supernatant with tap water and adjust the COD to 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 3% amylase according to the weight unit, stir evenly, carry out hydrolysis under the condition of 45-55 DEG C, the hydrolysis time is 72 hours, and obtain the reducing sugar solution with 60-80g / l reducing sugar. Add 2% active medical stone particles (particle size: 2-10mm) to the reducing sugar solution, soak at room temperature for 8 hours to decolorize, and precipitate minerals K, Ca, Na, P, Se, etc., after suction filtration Obtain a clear and transparent supernatant, then add 2% yeast extract, and stir evenly to obtain a Xanthomonas polysaccharide medium. Insert Xanthomonas campestris into the culture medium, the inoculum size is 2.0×10 per milliliter ...

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Abstract

The invention discloses a Xanthomonas amylose culture medium, a method for preparing the culture medium and the application of the culture medium in the production of Xanthomonas amylose; the raw materials used for preparing the culture medium comprises starch waste water, amylase, active medical stone granule and yeast extract; the preparation method comprises the steps of wastewater disposal, pH value control, zymohydrolysis and saccharification, as well as depigmentation and adding nutrientsubstance; the method has the advantages that the starch waste water is transformed into the culture medium for producing the Xanthomonas amylase, thus greatly reducing the production cost and realizing the technological innovation of the industry of the Xanthomonas amylose; meanwhile, the method can turn harm into good and change waste into valuable, so as to reduce the pollution of organic wastewater of starch for the environment and develop circular economy in 'second panning' of the starch waste water.

Description

technical field [0001] The invention relates to a culture medium for preparing xanthomonas polysaccharides, a preparation method of the culture medium, and an application of the culture medium in producing xanthomonas polysaccharides. Background technique [0002] Xanthan gum, also known as xanthan gum, is a single-spore polysaccharide produced by the fermentation of Pseudomonas spp. This bacterial exopolysaccharide has an ultra-high molecular weight, which is both biocompatible and biodegradable. Since the product was born in the 1950s, it has been widely used in more than 20 fields such as food, petroleum, and environmental protection. It is currently the microbial polysaccharide with the largest production scale and expanding uses in the world. [0003] The production scale of Xanthomonas polysaccharides needs to be continuously developed and expanded. Of course, we must also follow several basic principles of modern industrial development, especially the "principle of r...

Claims

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Application Information

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IPC IPC(8): C12P19/04C02F3/02C12R1/64C02F103/26
CPCY02W10/10
Inventor 金翼张艳红富昱金夕梦高诗语
Owner 金翼
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