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Detection chip for early diabetes peripheral nerve pathological changes and preparation and application thereof

A peripheral neuropathy and detection chip technology, applied in biological testing, material inspection products, etc., can solve the problems of difficult early diagnosis of diabetic peripheral neuropathy, complex molecular structure and anisotropy, and the difficulty of effective fixation, etc., to achieve Simple detection method, high sensitivity and low cost

Inactive Publication Date: 2009-02-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the preparation and analysis of protein chips also face more challenges
First of all, due to the limitation of serum protein concentration, the detection sensitivity is difficult to meet the requirements of applied research; secondly, the complex molecular structure and anisotropy of the protein itself add difficulties to the effective immobilization of the protein on the chip; in addition, multiple analysis Simultaneous analysis of objects also puts forward requirements for the development of new analysis models
[0005] In the present invention, a detection chip for clinical diagnosis of diabetic peripheral neuropathy is constructed by utilizing the clinically diagnostic landmark proteins COPE, DEFA1, NOTCH1 and BCL-6 in the peripheral serum of patients with diabetic peripheral neuropathy identified by mass spectrometry. By optimizing the test conditions, the requirements of effectively immobilizing the protein antibody to be tested on the substrate, stable quality control, standardized data analysis, and high reliability of test results are achieved, providing a non-invasive, sensitive and specific test for diabetic peripheral neuropathy. It is a screening method with good sex to solve the problem that the existing diabetic peripheral neuropathy is not easy to be diagnosed early and the detection cost is high

Method used

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  • Detection chip for early diabetes peripheral nerve pathological changes and preparation and application thereof
  • Detection chip for early diabetes peripheral nerve pathological changes and preparation and application thereof
  • Detection chip for early diabetes peripheral nerve pathological changes and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]In the first step, the concentration of the agarose solution is 1.0wt%, and the mixed solution sealed on the glass slide 11 is 1.0ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 10 μg / ml; in the third step, m=3; in the fourth step, the side length of the silicone rubber frame 4 is 0.2 mm.

Embodiment 2

[0047] In the first step, the concentration of the agarose solution is 1.5wt%, and the mixed solution sealed on the glass slide 11 is 2.5ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 100 μg / ml; in the third step, m=5; in the fourth step, the side length of the silicone rubber frame 4 is 5 mm.

Embodiment 3

[0049] In the first step, the concentration of the agarose solution is 2.0wt%, and the mixed solution sealed on the glass slide 11 is 4.0ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 200 μg / ml; in the third step, m=7; in the fourth step, the side length of the silicone rubber frame 4 is 10 mm.

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Abstract

Disclosed is a detecting chip for early stage diabetic peripheral nerve lesion, which comprises a detecting substrate, a reaction cell and a detecting microarray. The substrate is the glass slide whose surface is deposited with agarose-gel film, the reaction cell is arranged on the surface of the agarose-gel film, the reaction cell is formed by hollow square silastic frame surrounding around, the detecting microarray is dotted on the surface of the reaction cell, the size of the detecting microarray is five rows multiplied by m arrays, wherein, m is a natural number within 3 to 7, the component of the protein micro-solid-phase detecting points in each row is the same, the five rows of the protein micro-solid-phase detecting microarray points respectively are HIgG protein micro-solid-phase detecting points, Mc against COPE protein micro-solid-phase detecting points, Mc against DEFA1 protein micro-solid-phase detecting points, Mc against NOTCH1 protein micro-solid-phase detecting points and Mc against BCL-6 protein micro-solid-phase detecting points. The method of preparing the detecting chip is also provided. The detecting chip is particularly applied to detection of early stage diabetic peripheral nerve lesion.

Description

technical field [0001] The invention relates to a detection chip for early diabetic peripheral neuropathy and its preparation and application, belonging to the technical field of molecular diagnosis of medical equipment. Background technique [0002] With the development of the economy, the change of people's lifestyle, and the aging of the population, the number of diabetic patients is increasing rapidly. Diabetic peripheral neuropathy is the main cause of disability and death in diabetes. Its diagnosis is currently mainly determined by clinical performance scoring combined with electrophysiological examination techniques. However, the above-mentioned existing methods are complicated to operate, and the accuracy of the examination is limited by the patient's cooperation. , and the sensitivity to early peripheral neuropathy is poor, it is difficult to become an effective screening method, and it cannot provide a high-throughput standardized test that meets the needs of early...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 刘志民汤玮邹俊杰赵瑛
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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