Detection chip for early diabetes peripheral nerve pathological changes and preparation and application thereof
A peripheral neuropathy and detection chip technology, applied in biological testing, material inspection products, etc., can solve the problems of difficult early diagnosis of diabetic peripheral neuropathy, complex molecular structure and anisotropy, and the difficulty of effective fixation, etc., to achieve Simple detection method, high sensitivity and low cost
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Embodiment 1
[0045]In the first step, the concentration of the agarose solution is 1.0wt%, and the mixed solution sealed on the glass slide 11 is 1.0ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 10 μg / ml; in the third step, m=3; in the fourth step, the side length of the silicone rubber frame 4 is 0.2 mm.
Embodiment 2
[0047] In the first step, the concentration of the agarose solution is 1.5wt%, and the mixed solution sealed on the glass slide 11 is 2.5ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 100 μg / ml; in the third step, m=5; in the fourth step, the side length of the silicone rubber frame 4 is 5 mm.
Embodiment 3
[0049] In the first step, the concentration of the agarose solution is 2.0wt%, and the mixed solution sealed on the glass slide 11 is 4.0ml; in the second step, dilute HIgG, Mc anti-COPE, Mc anti-DEFA1, Mc anti-NOTCH1 and Mc anti-BCL-6 to a concentration of 200 μg / ml; in the third step, m=7; in the fourth step, the side length of the silicone rubber frame 4 is 10 mm.
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