Preparation of soy protein isolate

A technology of soybean protein isolate and soybean, which is applied in the field of preparation of soybean protein isolate, and can solve the problems of low beany smell and light color

Inactive Publication Date: 2009-02-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to use hydrothermal treatment of low-temperature vacuum-dried alcohol-processed soybean protein concentrate to improve its solubility more significantly than previous modification treatments, and to provide a method for extracting soybeans from alcohol-process soybean protein concentrate A

Method used

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  • Preparation of soy protein isolate

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Experimental program
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Effect test

Embodiment 1

[0024] Pass the defatted soybean flakes through a 20-mesh inspection sieve, move the sieved material into a container, soak it with 65% ethanol at a solid-to-liquid ratio of 1:5, extract it in four stages of countercurrent, and filter off the filtrate. The raffinate is placed in a vacuum drying oven and continuously dried at 40°C with a vacuum degree of 0.1 bar or higher for 2 hours, then taken out, and the dried soybean protein concentrate is crushed with a pulverizer, passed through a 200-mesh sieve, and stored in bags to obtain vacuum-dried alcohol French soy protein concentrate powder.

[0025] The obtained concentrated soybean protein powder was dispersed in the aqueous solution with a solid-to-liquid ratio of 1:10, and the pH value was adjusted to 8.0 with 2 mol / L sodium hydroxide. The solution was heated to 60 °C, kept at this temperature for 1 min and then cooled to room temperature.

[0026] The resulting solution was adjusted to pH 4.5 with 2mol / L hydrochloric acid,...

Embodiment 2

[0029] Pass the defatted soybean flakes through a 20-mesh inspection sieve, move the sieved material into a container, soak it with 75% ethanol at a solid-to-liquid ratio of 1:4, extract 8 stages of countercurrent extraction, and filter off the filtrate. The raffinate is placed in a vacuum drying oven and continuously dried at a vacuum degree of 0.1 bar or higher at 55°C for 1 hour, then taken out, and the dried soybean protein concentrate is crushed with a pulverizer, passed through a 100-mesh sieve, and stored in bags to obtain vacuum-dried alcohol French soy protein concentrate powder.

[0030] The obtained concentrated soybean protein powder was dispersed in the aqueous solution with a solid-to-liquid ratio of 1:15, and the pH value was adjusted to 9.0 with 2 mol / L sodium hydroxide. The solution was heated to 90 °C, kept at this temperature for 1 s and then cooled to room temperature.

[0031] Adjust the pH of the resulting solution to 3.0 with 2mol / L hydrochloric acid, a...

Embodiment 3

[0034] Pass the defatted soybean flakes through a 20-mesh inspection sieve, move the sieved material into a container, soak it with 70% ethanol at a solid-to-liquid ratio of 1:3, extract 6 stages of countercurrent extraction, and filter the filtrate. The raffinate is placed in a vacuum drying oven and continuously dried at 80°C with a vacuum degree of 0.1 bar or higher for 1 hour, then taken out, and the dried soybean protein concentrate is crushed with a grinder, passed through a 200-mesh sieve, and stored in bags to obtain vacuum-dried alcohol French soy protein concentrate powder.

[0035] The obtained concentrated soybean protein powder was dispersed in the aqueous solution with a solid-to-liquid ratio of 1:20, and the pH value was adjusted to 10.0 with 2 mol / L sodium hydroxide. The solution was rapidly heated to 141° C. in a pressure reactor, kept at this temperature for 30 minutes, and then cooled to room temperature.

[0036] Adjust the pH of the resulting solution to ...

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Abstract

The invention provides a method for preparing separated soy protein and comprises the following steps: (1) the preparation of concentrated soybean albumen powder by vacuum drying alcoholic method; (2) solution preparation and heat treatment; (3) acid coprecipitate and neutrality adjustment; (4) instantaneous heating and sterilizing as well as sponging drying. The separated soy protein prepared by the method not only has the advantages of high solubility and high protein content of common protein isolate, but also has the advantages of low beanyflavor, light color, low activity of nutrition resistant substance, easy treatment on discharged sewage, and the like which are not possessed by common separated soy protein; therefore, the invention is an ideal preparation method for producing beverage type separated soy protein.

Description

technical field [0001] The invention relates to a method for preparing soybean protein isolate, in particular to a method for preparing soybean protein isolate for further separating and extracting protein from alcohol-processed soybean protein concentrate. Background technique [0002] The traditional soy protein isolate is extracted from defatted soy flakes by using the alkali-dissolution-acid-precipitation method based on the change of soy protein solubility with the pH value of the solution. In the production process, it is usually necessary to use a solid-liquid ratio of 1:10-15 for multiple extractions to ensure a high extraction rate. Therefore, the production of 1 ton of protein isolate usually produces 20-30 tons of waste water, and this waste water is difficult to use. method processing. This is mainly because during the alkali dissolution process, the whey protein and soluble polysaccharide components of the defatted soy flakes are also dissolved, but they cannot...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 杨晓泉郑恒光齐军茹
Owner SOUTH CHINA UNIV OF TECH
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