Unlock instant, AI-driven research and patent intelligence for your innovation.

Novel antineoplastic compound Ananaside B in sea cucumber

A technology of anti-tumor drugs and compounds, applied in the field of medicine

Inactive Publication Date: 2009-03-04
宁波保税区欣诺生物技术有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical structure and antitumor activity of the saponins contained in it have not been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel antineoplastic compound Ananaside B in sea cucumber
  • Novel antineoplastic compound Ananaside B in sea cucumber
  • Novel antineoplastic compound Ananaside B in sea cucumber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The following examples further describe the separation and preparation of Ananaside B.

[0030] Wash 1000 grams of Plum Blossom Ginseng, chop it, soak it in turn with 5 times the amount (weight) of 95%, 85%, and 85% ethanol respectively, soak for 7 days each time, combine the ethanol extract, reclaim the ethanol under reduced pressure, and obtain the flow leaching paste. Dissolve the liquid extract in 3 times the amount of water, filter, and pass the filtrate through a DA201-B macroporous resin column, elute with water and 50% ethanol in sequence, collect the 50% ethanol eluate, recover ethanol under reduced pressure until dryness, and obtain total saponins 5.6 grams. The total saponins were subjected to silica gel column chromatography, eluted with a solvent of dichloromethane:methanol:water at a ratio of 7:3:1, and the fractions containing AnanasideB were collected, and then subjected to C 18 Reversed-phase column chromatography, gradient elution with methanol: wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a pharmaceutical technical field, particularly to an anticancer compound Ananaside B separated from thelenota ananas and having the molecular formula of C54H87O26SNa. The chemical construction and spatial configuration of the anticancer compound are determined by analyzing via multiple modern spectrums, particularly by applying comprehensive resolution of advanced two-dimensional nuclear magnetic resonance spectrum and high resolution mass spectrogram. An in vitro anti-tumor experiment indicates that the anticancer compound can obviously inhibit ten tumor cell strains such as A-549 lung cancer and IA-9 oophoroma. The anticancer compound provides a lead compound for the research of new antitumor drugs, and has important value on the exploitation and the utilization of ocean medicinal biological resources.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new antitumor compound Ananaside B isolated from marine animal ginseng. Background technique [0002] Plum ginseng (Thelenota ananas J.), also known as "pineapple ginseng", belongs to the sea cucumber family Aposticaceae of the marine echinodermata. Its body is very large, generally 700mm in length, 100mm in width, and 80mm in height. It is widely distributed in my country's Xisha Islands and the southern tip of Taiwan. Its body is rich in protein, polysaccharides, vitamins, and saponins, which have the effects of nourishing the liver and kidney, benefiting essence, and nourishing blood. The chemical structure and antitumor activity of the saponins contained in it have not been reported so far. Contents of the invention [0003] The present invention extracts and separates a new saponin compound from plum blossom ginseng grown in the sea area of ​​Fujian, China, named Ananaside B, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00A61K31/7048A61P35/00
Inventor 巫军
Owner 宁波保税区欣诺生物技术有限公司