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Biological chip glass carrier and preparation thereof

A technology of biochips and glass slides, applied in the field of biochip slides and its production, can solve problems such as difficult to achieve, obvious frame effect, low background, etc.

Active Publication Date: 2009-03-25
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the biochip slides produced are usually transparent glass slides and quartz slides. Although these two slides can be used in the production of biochips, they can achieve good uniformity, but their shortcomings are also very large. Obviously, first, it is difficult to achieve a very low background (even if it can be achieved, it is obtained under the premise of sacrificing the target signal strength); second, the border effect is obvious (chemiluminescence ELISA), which affects the analysis of edge data

Method used

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  • Biological chip glass carrier and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) under room temperature conditions, first the quartz sand that is 47.8% by weight and the sodium carbonate that is 28.9% by weight are mixed;

[0020] (2) Add 12% MnO2, 1% borax, 10% NaNO3, 0.1% carbon powder, 0.1% arsenic trioxide, 0.1% K2CO3, stir and mix;

[0021] (3) Put the above mixture into the melting furnace, heat and melt into a slurry, and continuously stir for 1 hour;

[0022] (4) Rapid cooling to 30°C;

[0023] (5) slice;

[0024] (6) Polishing and forming.

Embodiment 2

[0026] (1) under room temperature conditions, first the quartz sand that is 50.9% by weight and the sodium carbonate that is 30.6% by weight are mixed;

[0027] (2) Add 10% MnO2, 2% borax, 5% NaNO3, 0.5% carbon powder, 0.5% arsenic trioxide, 0.5% K2CO3, stir and mix;

[0028] (3) Put the above mixture into the melting furnace, heat and melt into a slurry, and continuously stir for 1 hour;

[0029] (4) Rapid cooling to 35°C;

[0030] (5) slice;

[0031] (6) Polishing and forming.

Embodiment 3

[0033] (1) under room temperature conditions, first the quartz sand that is 50.9% by weight and the sodium carbonate that is 30.6% by weight are mixed;

[0034] (2) Add 8% MnO2, 5% borax, 2.5% NaNO3, 1% carbon powder, 1% arsenic trioxide, 1% K2CO3, stir and mix;

[0035] (3) Put the above mixture into the melting furnace, heat and melt into a slurry, and continuously stir for 1 hour;

[0036] (4) Rapid cooling to 40°C;

[0037] (5) slice;

[0038] (6) Polishing and forming.

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Abstract

The invention discloses a glass slide of a biochip and a manufacture method thereof. The technical proposal is as follows: the glass slide of the biochip consists of the following ingredients by weight percentage: 44.7 percent to 50.9 percent of quartz sand, 28.9 percent to 46.25 percent of sodium carbonate, 0.1 percent to 12 percent of MnO2, 1 percent to 5 percent of borax, 0.1 percent to 10 percent of NaNO3, 0.1 percent to 2 percent of carbon powder, 0.1 percent to 2 percent of arsenic trioxide and 0.1 percent to 2 percent of K2CO3. The manufacture method of the glass slide of the biochip comprises the following steps: (1) under the condition of room temperature, the quartz sand and the sodium carbonate are mixed to be uniform firstly; (2) MnO2, the borax, NaNO3, the carbon powder, the arsenic trioxide and K2CO3 are added and stirred to be uniform; (3) the obtained mixture is thrown into a furnace, is heated, melted into slurry and stirred continuously for 1 hour; (4) the temperature is fast cooled to 25 to 40 DEG C; (5) slicing is carried out; and (6) polishing is carried out and then the glass slide of the biochip is formed. The film base of the background value of the glass slide is superior to the film base of the common glass slide obviously, and as to the signal strength, the average signal strength of the glass slide is superior to the common glass slide.

Description

technical field [0001] The invention relates to a biochip slide glass and a manufacturing method thereof. Background technique [0002] At present, the biochip slides produced are usually transparent glass slides and quartz slides. Although these two slides can be used in the production of biochips, they can achieve good uniformity, but their shortcomings are also very large. Obviously, first, it is difficult to achieve a very low background (even if it can be achieved, it is obtained under the premise of sacrificing the target signal strength); second, the border effect is obvious (chemiluminescence ELISA), which affects the analysis of edge data. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a biochip slide glass and its manufacturing method which can overcome the shortcomings of the prior art. [0004] The technical scheme of the present invention comprises that the biochip slide is made up of the following...

Claims

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Application Information

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IPC IPC(8): C03C6/04
Inventor 吴堂明潘能科陆冬雷张必达江晨亮
Owner 江苏三联生物工程股份有限公司
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