The invention discloses a glass slide of a biochip and a manufacture method thereof. The technical proposal is as follows: the glass slide of the biochip consists of the following ingredients by weight percentage: 44.7 percent to 50.9 percent of quartz sand, 28.9 percent to 46.25 percent of sodium carbonate, 0.1 percent to 12 percent of MnO2, 1 percent to 5 percent of borax, 0.1 percent to 10 percent of NaNO3, 0.1 percent to 2 percent of carbon powder, 0.1 percent to 2 percent of arsenic trioxide and 0.1 percent to 2 percent of K2CO3. The manufacture method of the glass slide of the biochip comprises the following steps: (1) under the condition of room temperature, the quartz sand and the sodium carbonate are mixed to be uniform firstly; (2) MnO2, the borax, NaNO3, the carbon powder, the arsenic trioxide and K2CO3 are added and stirred to be uniform; (3) the obtained mixture is thrown into a furnace, is heated, melted into slurry and stirred continuously for 1 hour; (4) the temperatureis fast cooled to 25 to 40 DEG C; (5) slicing is carried out; and (6) polishing is carried out and then the glass slide of the biochip is formed. The film base of the background value of the glass slide is superior to the film base of the common glass slide obviously, and as to the signal strength, the average signal strength of the glass slide is superior to the common glass slide.