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Culture medium for producing staurosporine and method thereof

A staurosporine and culture medium technology, which is applied in the field of culturing staurosporine-producing bacteria to produce staurosporine culture medium, which can solve the problems of mycelia easy to agglomerate, slow growth of bacteria, and fermentation culture Low product yield and other problems, to achieve the effect of not easy to agglomerate and fast growth

Inactive Publication Date: 2009-04-01
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention discloses a new compound STS produced by Streptomyces staurospora and its preparation method. However, when using this method to produce STS, the growth of the bacteria is slow, the hyphae are easy to agglomerate, and the yield of the fermentation product is low.

Method used

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  • Culture medium for producing staurosporine and method thereof
  • Culture medium for producing staurosporine and method thereof
  • Culture medium for producing staurosporine and method thereof

Examples

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Embodiment 1～30

[0036] The fermentation method is the same as above, except that the fermentation medium is replaced by the medium in the table below, and the other conditions are the same. The pH of the culture medium is 7.0, and the calcium carbonate is 0.4%.

[0037] Relative potency results: the peak area of ​​the culture medium product of Examples 1-30 measured by HPLC÷the above-mentioned standard product peak area)×100%, as shown in Table 1.

[0038] The medium is prepared by mixing and dissolving the ingredients in water.

[0039] Formulation and relative potency of medium in table 1

[0040]

[0041]

[0042] Percentages in the foregoing examples are all percentages by weight, and the MgSO 4 ·7H 2 Percentages of O and calcium carbonate are calculated as magnesium and calcium ions.

[0043] Drugs and reagents used in the above examples are:

[0044] Soluble starch, MgSO 4 ·7H 2 O: Sinopharm Chemical Reagent Co., Ltd.;

[0045] Yeast paste: Sinopharm Chemical Reagent Co., ...

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Abstract

The invention discloses a culture medium which is applicable to culturing staurosporine producing bacteria for producing staurosporine; the culture medium with pH value of 7.0 comprises carbon sources, organic nitrogen sources and inorganic salt, wherein, the inorganic salt comprises calcium salt; the carbon sources are selected from two or more than two of the following three groups of components, namely, A, B and C, wherein, at least two components are selected from different groups, group A: glucose, group B: amidulin, corn starch and potato starch, and group C: oats and maltodextrin; and / or, the organic nitrogen sources are selected from soybean cake powder, yeast extract, yeast cream and kapok meal; and / or, the inorganic salt is also added with magnesium salt. The invention also discloses a method for producing staurosporine from staurosporine producing bacteria by adopting the culture medium. By adopting the fermentation medium for producing staurosporine, the thalli of the staurosporine producing bacteria grows fast, the mycelia are not easy to clot, the yield of fermentation products is high, and the relative potency is optimized 230 percent of a former culture medium.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a medium for cultivating staurosporine-producing bacteria to produce staurosporine and a method thereof. Background technique [0002] Staurosporine (hereinafter referred to as STS) was isolated from the fermentation product of Saccharothrix staurosporeus AM-2282 by Japanese scholar S.QMURA in 1977 (Omura S, Iwai Y, Hirano A, et al. A new alkaloid AM-2282 of Streptomyces origin: taxonomy, fermentation, isolation and preliminary characterization. J. Antibiot, 1977, 30(4): 275-282), and later in Actinomycetes (Actinomycetes) family Streptomyces genus Streptomyces hygroscopicus, Streptomyces actuosus; STS was also obtained from the fermentation products of Saccharothrix, Actinomadura, Nocardia and other bacteria. The chemical structural formula of STS is shown below. [0003] [0004] STS is a broad-spectrum nonspecific protein kinase C inhibitor that potently inhibits ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12P17/18
Inventor 张海红胡海峰张琴
Owner SHANGHAI INST OF PHARMA IND CO LTD
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