Composition for treating bacterial, virus, fungal disease, inflammation and ache
A composition and extract technology, applied in the fields of viruses, fungal diseases, and bacteria treatment, can solve problems such as limited impact, inability to stabilize cell membranes, and insufficient
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preparation example Construction
[0015] The preparation of organic solvent extracts involves the preparation of a reaction product followed by extraction into a water-immiscible organic solvent to stabilize the reaction product.
[0016] The preparation of the reaction product involves first mixing methionine with water until a suspension is formed. Methionine may be L-methionine, D-methionine or a mixture thereof. Mixing is preferably performed under an inert gas such as argon. Mixing times are typically seconds to minutes. Mixing can be done by any mixing means, for example by a blender.
[0017] An aqueous solution of a hypochlorite such as sodium hypochlorite is added to the methionine solution and mixed well. The reaction mixture was then left to stand until the mixture was clear and excess methionine foamed on top. The foam can optionally be removed. The reaction is carried out at a temperature ranging from 0°C to ambient temperature, preferably at a low temperature such as 0-5°C. The reaction is ...
Embodiment 1
[0032] Embodiment 1. The preparation of product
[0033] Combine 720-750 ml of distilled water at 0-4° C. and 20 g of finely powdered DL methionine (Degussa Corp., Richfield Park, NJ) in a blender and place under argon Mix under air for a few seconds. After the methionine solids are uniformly suspended in distilled water, add 370-400ml of 0-4°C Clorox bleach (6% sodium hypochlorite) and mix at a blending rate under argon 30 seconds. Let the reaction sit for 30 seconds - 1 minute until the solution is clear and foam forms on top. The foam is excess methionine and can optionally be removed.
[0034] Immediately (<2 minutes) the solution was poured into an extractor blender, 240 ml of mineral oil (STO Oil Corp., San Marcos, TX) was added at room temperature, and then Blend on low speed for 20 seconds.
[0035] Let the water-oil mixture settle for 3-4 hours, then remove the oil phase and filter through 20 micron filter paper. The filtrate was stored in an open container for 3 day...
Embodiment 2
[0036] Embodiment 2. liver toxicity test
[0037] In vivo evaluation of the hepatotoxicity of the product of Example 1 was performed in rats.
[0038] A control group of 10 rats was left untreated for baseline serum enzyme and histological control analysis.
[0039] The second group of 10 rats was gavaged twice a day with standard saline solution to determine the effect of repeated operations and gavage on the liver.
[0040] The third group of 25 rats was fed with mineral oil alone twice a day.
[0041] The fourth group of 25 rats was gavaged twice a day with the product of Example 1.
[0042] After 28 days, the rats were anesthetized and their livers were examined according to standard methods. As a result, ingestion of the product of Example 1 did not cause any signs of hepatotoxicity.
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