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Mass spectrogram model for detecting brain glioma characteristic and preparing method thereof

A technology of glioma and characteristic protein, which is applied in the field of malignant tumor detection, achieves high sensitivity and specificity, reasonable and feasible design, and improves survival rate and quality of life

Active Publication Date: 2009-04-22
BIOYONG TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the accuracy of MALDI-TOF is as high as 0.1% to 0.01%, which is much higher than the current routinely used SDS electrophoresis and high-performance gel chromatography, there are still some problems in the application of tumor markers, especially glioma markers. Therefore, there is no report on the detection of glioma markers or glioma serum characteristic proteins using MALDI-TOF-MS technology in China so far.

Method used

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  • Mass spectrogram model for detecting brain glioma characteristic and preparing method thereof
  • Mass spectrogram model for detecting brain glioma characteristic and preparing method thereof
  • Mass spectrogram model for detecting brain glioma characteristic and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Establishment of glioma mass spectrometry model

[0036] 1. Samples and instruments:

[0037]A total of 147 serum samples were collected in two batches. The first batch of samples was 87 cases, of which 47 were from glioma patients and the other 40 were from healthy people. This batch of 87 samples was used as the training group. The second batch of samples was 60 cases, including 30 cases of glioma patients and 30 cases from healthy people. This batch of 60 samples was used as the test group. All 77 patients with glioma were confirmed by postoperative pathology report. All serum samples were drawn in the morning on an empty stomach, and the serum was separated and stored in a -80 low-temperature refrigerator.

[0038] The matrix-assisted laser desorption time-of-flight mass spectrometer Autoflex II TOF / TOF and the WCX magnetic bead kit used in the experiment were developed by Bruker Company of the United States. The data analysis software Clinprotools of...

Embodiment 2

[0056] Example 2 Detection of glioma

Embodiment 3

[0070] For the diagnosis of glioma, it is analyzed according to its biological characteristics, age, gender, predilection site and clinical process. On the basis of medical history and signs, electrophysiology, ultrasound, radionuclide, radiology and nuclear magnetic resonance are used to assist examine. In recent years, with the advancement of diagnostic imaging techniques and the application of microneurosurgery techniques, the diagnosis and treatment of gliomas have made significant progress in general. However, there seems to be no breakthrough in the treatment of malignant glioma, and most patients die within one year after diagnosis, and an earlier diagnosis method is urgently needed. However, the principle of the method of the present invention is different from the above detection methods, and the results of the method of the present invention show that the detection of glioma by serum polypeptide is also a very effective diagnostic method, which is of great benefit to...

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Abstract

The invention provides a mass spectrometry model used for detecting glioma serum characteristic protein and a preparation method thereof. The method uses a plurality of different characteristic protein combinations of glioma patients and normal people and detects the glioma serum, adopts a method combining the traditional statistics and the modern biological informatics to process data, measures the protein combination spectrogram of serum samples of glioma patients and normal people by a mass spectrograph, and screens out the tumor markers of 12 corresponding glioma characteristic proteins, thus preparing the mass spectrometry model used for detecting glioma serum characteristic protein and providing foundation and resource for finding newer and more ideal tumor markers.

Description

technical field [0001] The invention relates to the field of malignant tumor detection, and is a brand-new non-invasive detection method for early discovery and detection of brain glioma in vitro, and its sensitivity and specificity both reach more than 85%. Background technique [0002] Glioma is the most common malignant tumor of the central nervous system, accounting for about 46% of intracranial tumors. According to the data summarized in the third (Sydney) International Tumor Control Conference in 1996, the incidence of glioma is 3 to 10 / 100,000, accounting for 1% to 3% of systemic malignant tumors. The average survival period of surgery plus radiotherapy and chemotherapy is only 8 to 11 months. So far, there is no long-term follow-up data of large cases of glioma in my country. In 1998, the World Health Organization announced the ranking in order of mortality. Malignant glioma is the second cause of death for tumor patients under the age of 34, and the third cause of de...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N33/48
Inventor 殷汝雷马庆伟于中连胡晓慧张玮吕萍萍
Owner BIOYONG TECH
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