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Method for preparing paeoniflorin

A technology of paeoniflorin and paeoniflorin, which is applied in the field of production of paeoniflorin by extraction method, can solve the problems of unsatisfactory purification effect, high production cost, and low extraction efficiency, and achieve the effects of saving solvent consumption, easy control, and saving extraction time

Inactive Publication Date: 2009-04-29
ANHUI GUANGYINTANG CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing technologies mostly use conventional methods for extraction, with long heating time, low extraction efficiency, and high production costs; conventional separation methods such as macroporous resins are used for purification, which have low specificity and mostly unsatisfactory purification effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Preparation of reference substance solution: Accurately weigh an appropriate amount of paeoniflorin reference substance dried in a decompression desiccator with phosphorus pentoxide for 36 hours, add methanol to make a solution containing 0.5mg per 1ml, and obtain it.

[0029] Preparation of the test solution: Accurately weigh 12mg of the extract, put it in a 25ml measuring bottle, add methanol to dissolve and dilute to the mark, and shake well.

[0030] Determination method: Precisely draw 10 μl each of the reference substance solution and the test solution, inject it into a liquid chromatograph, measure it, and obtain it.

Embodiment 1

[0032] Put 1Kg of Radix Paeoniae Alba (derived from the dry root of Paeonia lactiflora Pall.) into the microwave extraction equipment, add 5L of water, and microwave once for 0.2 hours; collect the extract, filter it, and pass it through D101 large Load the sample on the porous adsorption resin column, first elute the impurities with water, then elute with 20% ethanol, collect 1 times the column volume of the ethanol eluate, recover the ethanol under reduced pressure and concentrate; On the chromatographic column, elute with water; collect the eluate to 1 times the column volume, concentrate through the reverse osmosis membrane, and dry to obtain 6.2 g of paeoniflorin with a content of 95.1%.

Embodiment 2

[0034] Put 1Kg of Radix Paeoniae Alba (from the dry root of Paeonia lactiflora Pall.) into the microwave extraction equipment, add 5L of water, microwave extraction 3 times, each time for 1 hour; collect the extract, filter, and make it pass through Load the D-3520 macroporous adsorption resin column, first elute the impurities with water, then elute with 80% ethanol, collect 8 times the column volume of the ethanol eluate, reclaim ethanol under reduced pressure and concentrate; the concentrated solution is added to On a chromatographic column equipped with activated carbon, elute with water; collect the eluate with 8 times the volume of the column, concentrate through a reverse osmosis membrane, and dry to obtain 11.2 g of paeoniflorin with a content of 94.8%.

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PUM

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Abstract

The invention relates to a method for preparing paeoniflorin, which is simple in operation and low in equipment investment. The method comprises the following process steps: a raw material containing paeoniflorin is placed into microwave extraction equipment and added with water for microwave extraction; filtrate is collected and filtered, so that the filtrate passes through a macroporous absorbent resin column for sample loading, water is used to elute impurities first, 20 to 80 percent ethanol is used for elution, ethanol eluate is collected, and the ethanol is decompressed, reclaimed and concentrated; the concentrated liquid is added into a chromatography column filled with active carbon, and then eluted by water; and eluate is collected, concentrated by a reverse osmosis membrane and dried to obtain the paeoniflorin. The method has the advantages of lowering production cost, improving product quality and facilitating industrialized operation.

Description

technical field [0001] The invention relates to a preparation method of paeoniflorin, in particular to a method for producing paeoniflorin by an extraction method. Background technique [0002] Paeoniflorin (paeoniflorin) is a monoterpene glycoside compound, which is widely found in various plants of Paeoniaceae, such as: Paeonialactiflora Pall., Paeonia lactiflora Pall.Var.trichocarpa (Bunge) Stern, Paeonia veitchii Lynch, Paeonia obovata Maxim., Paeonia obovata Maxim.var.willmottiae(Stapf) Stern, Paeonia mairei Lévl., Paeonia anomala L., Paeonia anomala L.var.intermedia (C.A.Mey.) O.et B.Fedtsh.., Paeonia suffruticosa Andr., Scrophularia ningpoensis Hemsl., Scrophularia buergeriana Miq., etc. Paeoniflorin has various physiological activities such as anti-inflammatory, analgesic, antithrombotic, neuroprotective, and hepatoprotective. In the prior art, conventional means are often used to extract the crude extract containing the substance from the raw material, and then fu...

Claims

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Application Information

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IPC IPC(8): C07H17/04
Inventor 高广印范淦彬
Owner ANHUI GUANGYINTANG CHINESE MEDICINE
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