Liver perfusate and preparation method thereof

A perfusion solution and liver technology, applied in the field of drugs for the treatment of liver damage, to increase safety and reliability, prevent damage to the liver, and reduce liver ischemia-reperfusion injury

Inactive Publication Date: 2009-05-20
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is no liver perfusate that can safely enter the human blood circulation and protect the liver from ischemia-reperfusion injury at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of liver perfusion solution, its raw materials and preparation tools are as follows: medical disposable syringe; disposable intravenous nutrition infusion bag (capacity 3000ml); sodium lactate Ringer's solution (500ml / bag, 4 bags in total, China Otsuka Pharmaceutical Co., Ltd. Co., Ltd.); reduced glutathione injection (2.4g, Chongqing Yaoyou Pharmaceutical Co., Ltd.); dexamethasone injection (30mg, Hubei Tianyao Pharmaceutical Company); verapamil injection (10mg, Datong Second Pharmaceutical Factory, Shanxi Province); 1,6-diphosphate fructose injection (22.5g, Biochemical Pharmaceutical Factory, Zhuhai Special Economic Zone, Guangdong Province).

[0027]The specific preparation method is as follows: under aseptic conditions, first inject 2000ml of sodium lactate Ringer's solution into the disposable intravenous nutrition infusion bag, and then inject the above-mentioned doses of reduced glutathione injection, dexamethasone injection, For verapamil i...

Embodiment 2

[0029] A preparation method of liver perfusion solution, its raw materials and dosage are as follows: sodium chloride 18g, potassium chloride 900mg, calcium chloride 600mg, sodium lactate 9300mg, 1,6-diphosphate fructose 15mmol, dexamethasone 30mg, reduced form Glutathione 9mmol and Verapamil 15mg.

[0030] Its specific preparation method is as follows: under sterile conditions, the sodium chloride, potassium chloride, calcium chloride, sodium lactate, 1,6-fructose diphosphate, dexamethasone, reduced glutathione, and vitamin After lapamil is dissolved in water for injection, mix homogeneously, be mixed with the product of the present invention of 3000ml; Above-mentioned sodium chloride, potassium chloride, calcium chloride, sodium lactate, 1,6-diphosphate fructose, dexamethasone, reduced glutathione Both Glycine and Verapamil are required to reach pharmaceutical grade.

Embodiment 3～5

[0032] A preparation method of liver perfusate, which is prepared into 3000ml of the product of the present invention, the specific preparation raw materials and steps are the same as in Example 2, the difference lies in the different amounts of each raw material, see Table 1 for the specific raw material dosage.

[0033] The consumption of each component in the table 1 embodiment 3~5

[0034] raw material name Example 3 Example 4 Example 5 Sodium chloride 5500mg / L 6000mg / L 6500mg / L potassium chloride 250mg / L 300mg / L 350mg / L calcium chloride 150mg / L 200mg / L 250mg / L sodium lactate 3100mg / L 3100mg / L 3100mg / L 1,6-Fructose diphosphate 4mmol / L 5mmol / L 6mmol / L Dexamethasone 15mg / L 10mg / L 15mg / L reduced glutathione 2.5mmol / L 3mmol / L 3.5mmol / L verapamil 4mg / L 5mg / L 6mg / L

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PUM

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Abstract

The invention provides a liver perfusate, which is prepared by 5,500 to 6,500 mg/L of sodium chloride, 250 to 350 mg/L of potassium chloride, 150 to 250 mg/L of calcium chloride, 3,000 to 3,200 mg/L of sodium lactate, 4 to 6 mmol/L of 1, 6-diphosphofructose, 5 to 15 mg/L of dexamethasone, 2.5 to 3.5 mmol/L of reduced glutathione, and 4 to 6mg/L of verapamil. The product has a simple formula, convenient configuration, low cost and proper potassium concentration, can be safely used for the preservation of isolated organs, more importantly can be applied to the perfusion protection of in vivo organs, and can effectively prevent the injury of hepatic ischemia reperfusion. The invention also provides two preparation methods for the liver perfusate.

Description

technical field [0001] The invention relates to a medicine for treating liver damage, in particular to a liver perfusate; the invention also relates to a preparation method of the liver perfusate. Background technique [0002] In order to control bleeding during liver lobectomy and radically cure various malignant tumors invading the portal vein / superior mesenteric vein, it is often necessary to completely or partially block the blood flow into the liver. Perfusion injury, especially on the basis of the original liver disease, can easily lead to postoperative hepatic insufficiency, or even liver failure, which seriously affects the postoperative recovery of patients. [0003] Liver cold perfusion technology has been widely used in the preservation of clinically transplanted donor livers and the protection of liver ischemia caused by hepatic blood flow occlusion during hepatic lobectomy. A large number of experimental studies and clinical applications have confirmed that it c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/06A61K33/14A61K31/19A61K31/573A61K31/275A61P41/00
Inventor 张雷达别平范毓东丁军李大江冯春林
Owner ARMY MEDICAL UNIV
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