Liver perfusate and preparation method thereof

Abstract

The invention provides a liver perfusate, which is prepared by 5,500 to 6,500 mg / L of sodium chloride, 250 to 350 mg / L of potassium chloride, 150 to 250 mg / L of calcium chloride, 3,000 to 3,200 mg / L of sodium lactate, 4 to 6 mmol / L of 1, 6-diphosphofructose, 5 to 15 mg / L of dexamethasone, 2.5 to 3.5 mmol / L of reduced glutathione, and 4 to 6mg / L of verapamil. The product has a simple formula, convenient configuration, low cost and proper potassium concentration, can be safely used for the preservation of isolated organs, more importantly can be applied to the perfusion protection of in vivo organs, and can effectively prevent the injury of hepatic ischemia reperfusion. The invention also provides two preparation methods for the liver perfusate.

Description

technical field

[0001] The invention relates to a medicine for treating liver damage, in particular to a liver perfusate; the invention also relates to a preparation method of the liver perfusate. Background technique

[0002] In order to control bleeding during liver lobectomy and radically cure various malignant tumors invading the portal vein / superior mesenteric vein, it is often necessary to completely or partially block the blood flow into the liver. Perfusion injury, especially on the basis of the original liver disease, can easily lead to postoperative hepatic insufficiency, or even liver failure, which seriously affects the postoperative recovery of patients.

[0003] Liver cold perfusion technology has been widely used in the preservation of clinically transplanted donor livers and the protection of liver ischemia caused by hepatic blood flow occlusion during hepatic lobectomy. A large number of experimental studies and clinical applications have confirmed that it c...