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Method for preparing stable coated granzyme

A granzyme and coating technology, applied in the direction of immobilization on/in the organic carrier, can solve the problems of low cost, unstable enzyme activity, easy mildew, etc.

Inactive Publication Date: 2009-05-27
ZHUHAI BAICO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to overcome the problems of large loss of enzyme activity in the production process of coated enzyme, unstable enzyme activity and easy mildew in long-term storage, and the production process is simple, easy to scale production, and low cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Put 1000g of high-concentration cellulase (500000BXU / g), 2000g of cornstarch, 500g of dextrin, 100g of dioxidized peptide, 1000g of trehalose, and 20g of calcium propionate into a mixer, mix them evenly, add water to make a wet material, put Wet granules are prepared in a oscillating granulator; put the wet granules into the coating pan and rotate and roll (speed 30r / min), and coat the coating solution (prepared by adding polyethylene glycol, calcium propionate, gelatin and appropriate amount of water) Spray evenly on the surface of the material, make wet pellets, put them into a boiling dryer at 30°C-45°C to dry for 1 hour, and sieve and classify the finished product in the range of 20-80 mesh. The measured moisture content of the finished product is 8%, and the enzyme activity The value is 107620BXU / g, and the enzyme activity preservation rate in the processing process is close to 100%.

[0022] The above products are sealed and vacuum-packed with plastic film, and st...

Embodiment 2

[0024] Put 1000g of high-concentration xylanase (1700000BXU / g), 3000g of cornstarch, 1000g of dextrin, and 20g of calcium propionate into a dry powder mixer, mix evenly, add water to make a wet material, and put it into a oscillating granulator to make a wet material. Granules, put the wet granules into the coating pot and rotate and roll (speed 40r / min), spray the coating liquid (prepared from polyethylene glycol, calcium propionate, starch, gelatin and appropriate amount of water) evenly on the surface of the material After being made into wet pellets, put them into a boiling dryer at 30°C-45°C to dry for 1.2 hours, and sieve and classify the finished product in the range of 20-80 mesh. The measured moisture content of the finished product is 7.5%, and the enzyme activity value is 335150BXU / g, the enzyme activity loss rate during processing is about 1%.

[0025] The above-mentioned products are sealed and vacuum-packed with plastic film, stored in an indoor environment of 1...

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PUM

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Abstract

The invention discloses envelope granzyme favorable for the normal temperature storage and a preparation method thereof. The envelope granzyme prepared by the preparation method is provided with an enzyme-containing granular layer and a special envelope layer, a formula contains a raw material, a filling agent, an adhesive, a stabilizer and a mildew inhibitor, and the vacuum packaging is adopted. The multiple protection increases the stability of enzyme, and a product can maintain higher enzyme activity level within one year at normal temperature. The envelope granzyme can be used for producing feed enzyme, food enzyme, industrial enzyme and so on.

Description

technical field [0001] The present invention is applicable to the production of coated granzyme, such as: cellulase, amylase, xylanase, phytase, lipase, protease etc., these coated granzymes can be respectively or compoundly used in feed industry, alcohol , brewing, printing and dyeing, daily chemical and other industries. Background technique [0002] Enzyme preparations have been widely used in feed industry, alcohol, brewing, printing and dyeing, daily chemical and other industries. Currently, the commonly used enzyme preparations are powder enzymes or liquid enzymes. Such products generally have problems of poor stability during storage and use. For example, powdered or liquid feed enzymes are easily inactivated in high-temperature granulation environments, and enzymes added to washing powder are easily volatile in alkaline environments. live. One of the methods to solve the aforementioned problems is to use coating technology, that is, to coat one or several layers of...

Claims

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Application Information

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IPC IPC(8): C12N11/04
Inventor 李丽娟
Owner ZHUHAI BAICO BIOTECH
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