Method for extracting beta-amylase from potato cell tissue

A technology of cell tissue and amylase, applied in the direction of hydrolytic enzymes, etc., to achieve the effects of good color, low cost, significant economic and social benefits

Inactive Publication Date: 2009-06-10
ZHENGZHOU JINTUDI ENERGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, up to now, do not see the bibliographical information about extracting β-amylase from the supernatant liquid discharged from sweet potato processing production, the inventor has developed a kind of method for extracting β-amylase from sweet potato cell tissue through long-term research, Created a new production process for the domestic production of β-amylase

Method used

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  • Method for extracting beta-amylase from potato cell tissue

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A method for extracting β-amylase from potato cell tissue

[0033] The detailed steps of this method are as follows:

[0034] See attached figure 1 , the prepared sweet potatoes are cleaned in the cylinder machine, and after cleaning, they are crushed by adding water to the file grinder, and the crushed slurry residue is centrifugally separated, and the wet residue discharged after separation is sold, and the slurry is separated by cyclone, separated Impurities, crude fibers and yellow powder are removed, and the refined starch liquid obtained after cyclone separation is sucked and filtered by a vacuum suction filter. The wet starch is bonded to the outer layer of the 120-7 filter cloth, and the clear liquid flows out. The clear liquid is further processed to extract β - Amylase, the following detailed steps are:

[0035] a. The clear liquid obtained after the suction filtration is introduced into the decolorizer, and an activated carbon decolorizer accoun...

Embodiment 2

[0039] Embodiment two: a kind of method for extracting β-amylase from tuber cell tissue, basically the same as embodiment one, the difference is:

[0040] The decolorizer that adopts in the step a is gac, and its add-on accounts for 2.5% of clear liquid amount;

[0041] In step b, three filter adsorptions of 300# diatomite, a mixture of 300# and 600# diatomite, and 600# diatomite uniformly pre-coated on the 120-10 filter cloth,

[0042] The thickness of the polyethersulfone membrane in step c is 20 microns.

Embodiment 3

[0043] Embodiment three: a kind of method for extracting β-amylase from tuber cell tissue, basically the same as embodiment one, the difference is:

[0044] The decolorizing agent adopted in the step a is gac, and its addition accounts for 3% of clear liquid amount;

[0045] In step b, pass through the 300# diatomite evenly pre-coated on the 120-10 filter cloth, the mixture of 300# and 600# diatomite on the 120-7 filter cloth, and the 600# diatomite on the 120-10 filter cloth Three filter adsorption;

[0046] The thickness of the polyethersulfone membrane in step c is 18 microns.

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Abstract

The invention discloses a method for extracting beta-amylase from potato cell tissues. The method comprises: cleaning and crushing raw materials, centrifugally separating slurry residue, selling wet residue obtained after centrifugal separation, performing cyclone separation and suction filtration on serum, performing adsorption bleaching and filtration on a clear solution obtained after suction filtration, and finally adopting membrane separation technology to extract beta-amylase products from the filtered clear solution. The beta-amylase obtained by the method has the advantages of non-pollution, good color and luster, low cost and so on, is superior enzyme preparation for producing foodstuffs, can be widely applied in the beer industry and the malt syrup industry, and is a most ideal malt product. The method extracts the beta-amylase from waste liquid generated by production of sweet potato starch, changes the waste liquid polluting the environment into valuable substances, becomes a type of recycling economy, and has obvious economic benefit and social benefit.

Description

1. Technical field: [0001] The invention relates to a method for extracting beta-amylase, in particular to a method for extracting beta-amylase from potato cell tissue. 2. Background technology: [0002] β-amylase is widely found in barley, wheat, oat, soybean, sweet potato and other plants, and is an extracellular enzyme. β-amylase is a high-tech enzyme preparation product, which is widely used in the beer industry and malt sugar industry, and is the most ideal substitute for malt. [0003] β-amylase is produced abroad from Bacillus megaterium through submerged fermentation culture, extraction and other processes. The β-amylase produced has high activity and good thermal stability. [0004] In China, β-amylase is directly extracted from soybeans, which has the advantages of no pollution and good color. There are also literature reports about extracting β-amylase from sweet potato. But, up to now, do not see the bibliographical information about extracting β-amylase from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24
Inventor 吴得治
Owner ZHENGZHOU JINTUDI ENERGY TECH
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