Unlock instant, AI-driven research and patent intelligence for your innovation.

Methods of Analysis of Steroids

An analytical method and compound technology, applied in the direction of analytical materials, material separation, measuring devices, etc., to achieve the effects of good stability, good applicability, and simple and convenient operation.

Inactive Publication Date: 2011-12-14
TIANJIN JINYAO GRP
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no literature reports to solve this problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of Analysis of Steroids
  • Methods of Analysis of Steroids
  • Methods of Analysis of Steroids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Chromatographic conditions:

[0043] Chromatographic column: C18; 5μm; 4.6mm×250mm.

[0044] Mobile phase: acetonitrile: water = 43: 57 (volume ratio)

[0045] Injection volume: 50μl

[0046] Detection wavelength: 240nm

[0047] Flow rate: 1.0ml / min

[0048] Operation: Take an appropriate amount of sample, accurately weigh it, add acetonitrile to dissolve and quantitatively dilute to make a solution containing 0.4mg per 1ml, shake well, accurately measure 50μl and inject it into the liquid chromatograph, record the chromatogram, and obtain the normalization of the sample French liquid content.

[0049] Sample: the same batch number (060501) of halomethone and the intermediates involved in its synthesis, the liquid phase results are shown in the attached Figure 1-5 .

[0050] sample

Embodiment 2

[0052] Chromatographic conditions:

[0053] Chromatographic column: C18; 5um; 4.6mm×250mm.

[0054] Mobile phase: acetonitrile-water (4:6)

[0055] Injection volume: 50μl

[0056] Detection wavelength: 240nm

[0057] Flow rate: 1.0ml / min

[0058] Operation: Take an appropriate amount of 6 halomethasone samples, accurately weigh them, add acetonitrile to dissolve and quantitatively dilute to make a solution containing 0.8 mg per 1 ml, shake well, accurately measure 50 μl and inject it into a liquid chromatograph, and record the chromatogram; Normalized liquid phase content.

[0059] Sample 6 and sample 1 are the same batch of halomethone, the obtained liquid phase content: 98.96%, retention time (min): 17.732, see the attached HPLC figure Figure 6 .

Embodiment 3

[0061] Chromatographic conditions:

[0062] Chromatographic column: C18; 5μm; 4.6mm×250mm.

[0063] Mobile phase: acetonitrile-water (48:52)

[0064] Injection volume: 50μl

[0065] Detection wavelength: 245nm

[0066] Flow rate: 1.0ml / min

[0067] Operation: Take an appropriate amount of 7 halomethasone samples, accurately weigh them, add acetonitrile to dissolve and quantitatively dilute to make a solution containing 0.2mg per 1ml, shake well, accurately measure 50μl and inject it into the liquid chromatograph, and record the chromatogram; Normalized liquid phase content.

[0068] Sample 7 and sample 1 are the same batch of halomethone, the obtained liquid phase content: 99.06%, retention time (min): 9.107, the HPLC figure obtained is shown in the attached Figure 7 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

An analysis method for steroidal compounds, involving the analysis method of halomethone series compounds and some intermediates involved in their synthesis, providing a brand-new halomethone liquid phase analysis method, which can systematically analyze halomethone series compounds and its synthetic intermediates.

Description

technical field [0001] The present invention relates to the analysis method of halomethone series compounds and some intermediates involved in their synthesis. Background technique [0002] Halomethone is a powerful halogen-containing glucocorticosteroid drug for external use. It has rapid anti-inflammatory, anti-allergic, anti-itching, vasoconstrictive, anti-exudative and anti-proliferative effects. It has strong activity and fast action, and is widely used in different types of For inflammatory skin diseases, it can quickly reduce and eliminate symptoms such as itching. [0003] There have been many articles and reports about haloforminone, but there are only one article about the analysis method of its preparation, which is briefly introduced as follows: [0004] Literature Cesko-Slovenska Farmacie (1986), 35(6), 265-9. mentioned the use of differential pulse polarography (differential pulse polarography; DPP) method, halomethone ointment, cream and tablet content was m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/28
Inventor 朱润芝樊娜
Owner TIANJIN JINYAO GRP