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Method for preparing biodiesel from sugarcane

A biodiesel and sugarcane technology, applied in the preparation of biological raw materials, biofuels, liquid hydrocarbon mixtures, etc., can solve the problems of high cost and cumbersome steps, and achieve the effects of reducing production costs and simplifying the cultivation process.

Inactive Publication Date: 2012-05-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods require a complex medium preparation process, additional substances need to be added, the steps are cumbersome, the cost is high, and there are certain defects in large-scale industrial production.

Method used

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  • Method for preparing biodiesel from sugarcane
  • Method for preparing biodiesel from sugarcane
  • Method for preparing biodiesel from sugarcane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Preparation of seeds: Chlorella protothecoides (Chlorella protothecoides) (purchased from the Algae Center of the University of Texas, U.S.) is cultivated in a heterotrophic medium as seeds, and the heterotrophic medium includes: 30gL -1 Glucose, 3gL -1 Yeast extract, 0.7gL -1 K H 2 PO 4 , 0.3gL -1 K 2 HPO 4 , 0.3gL -1 MgSO 4 ·7H 2 O, 0.3mgL -1 FeSO 4 ·7H 2 O, 5gL -1 Glycine and 0.01mg L -1 Vitamin B1.

[0076] (2) Obtaining sugarcane juice enzymatic hydrolysis: use a commercially available household sugarcane juicer to obtain sugarcane juice (model YZ28, brand: Henglian), which has a hydraulic device inside, integrating rolling, squeezing and collecting operations, The sugarcane juice is squeezed directly through powerful pressure.

[0077] The sugarcane juice is extracted from fresh sugarcane bought in the market and collected in a container. Under the greenhouse, add sucrose invertase (trade name Validase) to sugarcane juice Invertase ANL), the ...

Embodiment 2

[0083] Experiment with the same method as in Example 1, except that the shaking flask culture of Chlorella is replaced with the following fermentation experiments:

[0084] 5L fermentation experiment of Chlorella: Prepare a 5L fermenter (MINIF ORS, Switzerland), assemble temperature, pH, dissolved oxygen electrodes and defoaming electrodes, add 3L heterotrophic medium as described in step 3, and steam sterilize Let cool and set aside. The heterotrophic fermentation conditions are set as follows: temperature 28±1°C, aeration rate about 180L / h, pH value 6.3±0.5, stirring speed 350±50rpm; online monitoring of dissolved oxygen changes in the fermenter to keep it at 10- Within the range of 40% saturation; when the carbon source is consumed, the concentrated enzymolysis solution described in Example 2 (for ease of operation, the enzymolysis solution is preferably concentrated to a reducing sugar concentration of about 500g / L through freeze-drying) flow Add it to the fermenter to co...

Embodiment 3-7

[0086] Experiment in the same way as in Example 1, except that the initial reducing sugar concentration in the shake flask is configured as: 5gL -1 , 10gL -1 , 15gL -1 , 25gL -1 , 30gL -1 outside. From Figure 7 It can be seen that within 75 hours of culture time, Chlorella can grow in different carbon source concentration ranges, showing a certain tolerance.

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Abstract

The invention relates to a method for producing biodiesel from sugarcane serving as a raw material, and is characterized in that the method comprises the following steps: (1)extracting sugarcane juice from the sugarcane; (2)degrading the polysaccharides in the sugarcane juice so that the carbon source therein is converted into a form suitable to be used by oil production microalgae to obtain a sugarcane juice degradation solution; (3)cultivating the oil production microalgae in the sugarcane juice degradation solution; (4)obtaining grease from the oil production microalgae; and (5) preparing the biodiesel from the grease. With the method, when the polysaccharides in the sugarcane juice are converted into monosaccharide and the sugarcane juice degradation solution is obtained, other nutrilits are not necessary to be added into the sugarcane juice degradation solution which is used for cultivating chlorella directly, thus not only the cultivating process is simplified, but also the production cost is reduced. The method is highly advantageous for large scale biomass production and grease production.

Description

technical field [0001] The invention belongs to the field of renewable bioenergy, and relates to a method for producing biodiesel with sugarcane as a raw material. Background technique [0002] With the rising global crude oil prices and the aggravation of the greenhouse effect, countries in recent years have stepped up research and development and promotion of biofuels. As a clean alternative fuel, biodiesel is biodegradable, non-toxic, almost free of sulfur and aromatic compounds, and easy to use. It can be used directly without modification of the engine or with only minor changes. Biodiesel and petrochemical diesel are blended in any proportion before use (Palligarnai T et al. Biodieselproduction-current state of the art and challenges. J IndMicrobiol Biotechnol. 2008). [0003] Biodiesel is a kind of biomass energy, and its production raw materials are very wide. It can not only use starch crops such as corn, cassava, sorghum, sugar crops such as sugar cane and sugar b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10G3/00C12P7/649
CPCC10L1/026Y02E50/13C12P7/649Y02P30/20Y02E50/10
Inventor 吴庆余程云
Owner TSINGHUA UNIV