Method for rapidly detecting inorganic phosphorus in water solution

Abstract

The invention provides a method for quickly detecting inorganic phosphorus in a water solution, which directly, qualitatively and quantificationally detects the inorganic phosphorus in a buffer solution with a pH value of 7.0 by taking 4-(2-pyridylazo)-resorcinol (PAR) as a visualization reagent based on divalent lead Pb<2+> ions. The method concretely comprises a spectrometry, a reagent kit method and a test paper method. The methods can be directly used for the determination of the inorganic phosphorus in environmental water sources, blood, urine, milk, cola, beer, and so on. The method hasthe advantages of simple detection process, intuitive and convenient discrimination, and easy popularization and promotion. The method has accurate measurement result and high sensitivity, is not interfered by other anions, can be promoted and applied to the determination of the inorganic phosphorus in clinical blood samples, urine samples, the environmental water sources, and food, and has larger market development value and application prospect.

Description

Method for Rapid Detection of Inorganic Phosphorus in Aqueous Solution Technical field: The invention relates to a detection technology for inorganic phosphorus, in particular to a method for rapidly detecting inorganic phosphorus in an aqueous solution. Background technique: Phosphorus is the most basic element in the synthesis of DNA and RNA, energy accumulation and conduction, and other biological reactions in the life system. It is one of the most abundant elements in the human body, second only to calcium. Eighty percent is combined with calcium and stored in bones and teeth, and the remaining 20 percent exists in cells as organic phosphorus such as phospholipids, nucleic acids, and ATP, or as inorganic phosphorus outside cells. Dietary phosphorus is thought to be a factor that impairs residual renal function in patients with chronic renal failure. Phosphorus is also involved in various important biomineralization processes, such as bone formation and pathological pr...

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Problems solved by technology

It should be pointed out that due to the narrow linear range of many instruments in clinical measurement of inorganic phosphorus, it needs a large degree of dilution of the sample to be measured; the use of synthetic receptors requires a lot of synthesis work, and the water solubility of the receptors is also limited. Can cause inconvenience to the measurement work; many colorimetric methods for the detection of inorganic phosphorus are either based on the further reduction of phosphomolybdic acid to heteropolymolybdenum blue, or on the direct determination of molybdenous and vanadous phosphomolybdic acids, or on the basis of phosphomolybdic acid and alkali Complexes of sexual dyes

These chemistries have serious drawbacks, namely: molybdate reduction is affected by slight pH changes, the rate of complex formation is strongly affected by protein concentration, and the required pH acidity will lead to the hydrolysis of organophosphates, which will lead to the measured High concentration of inorganic phosphorus

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