Tetracycline stringent controlled eucaryon expression vector
A technology of eukaryotic expression vector and tetracycline, which is applied in the field of molecular biology, can solve problems such as heavy workload, loss, and weakened vector expression, and achieve the effects of improving work efficiency, saving time and cost, and high sensitivity
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[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, and the described embodiments are explanations of the present invention rather than limitations of the present invention.
[0028] The tetracycline tightly controlled expression system of the present invention is based on the Tet open expression system. The elements required for Tet regulation are composed of two expression cassettes, one of which encodes a Tet or Dox response element, and the other encodes a repressor . Insert the repressor rtTA2 along the direction of the promoter S -M2 element, its sequence is shown in SEQ ID NO.1, encoding rtTA2 S - M2 protein; rtTA2 S -M2 is an optimized inhibitor of the tetracycline response element. In the absence of Tet and Dox, rtTA2 S -M2 protein inhibits the transcription of genes controlled by it by binding to tetracycline response expression elements (Tet or Dox response elements); and Tet and ...
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