Method for measuring melamine content in solid example

Abstract

A method for measuring the content of melamine in a solid sample uses a portable Raman spectrometer to directly measure Raman spectrum for the solid sample. The method specifically includes the following steps: 260 to 300 mg solid sample to be measured is weighed and loaded in a glass tube for testing, and the cover is closed; the solid sample is fully mixed for 2min on a turbine mixer and is inserted on a sample holder of the portable Raman spectrometer; and the Raman spectrum in the range of 450 to 1050 cm<-1> is recorded. The method is convenient, practical and economical without pretreatment process, thereby having quick measurement and being applicable to field high-flux detection; the method is portable, needs few consumption of sample, and can be qualitative and quantitative; and the method is particularly applicable to monitoring and detecting for high concentration melamine added milk source at milk source gathering stations.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for determining the content of melamine in a solid sample. Background technique [0002] Although melamine (2,4,6-triamino-1,3,5-triazine) itself is not a carcinogen, if illegal large doses of melamine are mixed in dairy products, it will likely lead to urinary stones, acute kidney disease failure, and even infant death, as in the 2007 incidents of melamine contamination in pet feed in the United States and in September 2008 in infant formula in China. Therefore, it is urgent to establish a rapid and accurate on-site detection method. [0003] Since the characteristic peak of the ultraviolet absorption spectrum of melamine is below 240nm, when the ordinary ultraviolet absorption detection method detects melamine, it is easily affected by the solvent and the sample matrix, resulting in inaccurate test results. Moreover, when traditional testing methods, such as HPLC-DAD, LC / MS / MS, ...

AI Technical Summary

Problems solved by technology

[0003] Since the characteristic peak of the ultraviolet absorption spectrum of melamine is below 240nm, when the ordinary ultraviolet absorption detection method detects melamine, it is easily affected by the solvent and the sample matrix, resulting in inaccurate test results.

Moreover, when traditional testing methods, such as HPLC-DAD, LC / MS / MS, GC / MS, and ELISA, are applied to the detection of melamine, complex and time-consuming pretreatment procedures are required, and the interference is serious, and the instrument takes up space Big

Therefore, these existing test methods are not suitable for the requirements of on-site rapid detection

Images