Active lactic acid bacteria beverage for pet and preparation method thereof
A technology of active lactic acid bacteria and lactic acid bacteria drinks, which is applied in the field of animal health care products to achieve the effects of improving pet immunity, lowering PH value, and reducing the production of toxic fermentation products and harmful bacterial enzymes
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Embodiment 1
[0032] Embodiment 1: the preparation of lactic acid bacteria beverage
[0033] Raw materials (percentage by weight): lactic acid bacteria, 1.5×10 9 CFU / 100ml, emulsion stabilizer, 0.5%, sodium carboxymethylcellulose, 0.6%, sodium benzoate, 0.5%, citric acid, 0.3%, lactose, 15%, essence, 0.8%.
[0034] The preparation process is as follows:
[0035] 1) After mixing the emulsification stabilizer and lactose, dissolve them completely in hot water at 70-80°C and keep warm for 30 minutes. Then cool to below 30°C.
[0036] 2) Pour the cooled mixed solution of emulsification stabilizer and lactose into the evenly stirred lactic acid bacteria, then stir to make it uniform, and add sodium benzoate.
[0037] 3) Mix citric acid and purified water into a 5% solution, and then slowly add it into the prepared lactic acid bacteria while stirring.
[0038] 4) Adjust the fragrance with essence, and add sodium carboxymethyl cellulose to adjust the viscosity.
[0039] 5) Carry out high-pres...
Embodiment 2
[0042] Embodiment 2: the preparation of lactic acid bacteria drink
[0043] Raw materials (percentage by weight): lactic acid bacteria, 1×10 9 CFU / 100ml, emulsion stabilizer, 0.3%, sodium carboxymethylcellulose, 0.5%, sodium benzoate, 0.5%, citric acid, 0.25%, lactose, 12%, flavor, 0.8%.
[0044] The preparation process is as follows:
[0045] 1) After mixing the emulsification stabilizer and lactose, dissolve them completely in hot water at 70-80°C and keep warm for 30 minutes. Then cool to below 30°C.
[0046] 2) Pour the cooled mixed solution of emulsification stabilizer and lactose into the evenly stirred lactic acid bacteria, then stir to make it uniform, and add sodium benzoate.
[0047]3) Mix citric acid and purified water into a 4% solution, and then slowly add it into the prepared lactic acid bacteria while stirring.
[0048] 4) Adjust the fragrance with essence, and add sodium carboxymethyl cellulose to adjust the viscosity.
[0049] 5) Carry out high-pressure h...
Embodiment 3
[0052] Embodiment 3: the animal toxicological test of lactic acid bacteria beverage
[0053] 1. Test materials and methods
[0054] 1.1 Active lactic acid bacteria beverage for pets is prepared according to Example 1 of the present invention, and its main components are lactic acid bacteria, emulsion stabilizer, thickener, sodium benzoate, acidity regulator, lactose, and essence. Do not dilute before use, for oral drink.
[0055] 1.2 Experimental animals 20 mice, male and female, weighing 18g-22g, were provided by R&D Experimental Animal Center of Ringpu Corporation.
[0056] 1.3 Toxicology test: Divide the mice into groups, half male and half female, 10 as the test drug group, and the other 10 as the negative control group, fasting before the experiment, and after 12 hours without water, take 30mL of lactic acid bacteria pet drink, according to the weight of the test animals , according to the maximum volume of 3mL / 10g (equivalent to twice the adult drinking amount) orally....
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