Production technique of dual vaccine of chicken new castle disease and infectious bronchitis

A technology for bronchitis and chicken Newcastle disease, applied in the field of chicken Newcastle disease, can solve the problems of reduced antigen titer of antigen composition, simple production process of multiple vaccines, mutual interference of immune responses, etc., so as to reduce the number of immunizations, improve the quality of immunization, and avoid serious interference. Effect

Inactive Publication Date: 2009-07-29
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many varieties of multiple vaccines for veterinary use in my country, such as "Newcastle disease, infectious bronchitis dual vaccine", "Newcastle disease, infectious bronchitis, bird flu triple vaccine" and so on. Although there are many varieties, many multiple vaccines It is just a simple combination of multiple single vaccines, the ratio of several antigens is chaotic, the compatibility is not good, and there is a phenomenon of mutual interference of immune responses, which cannot achieve the expected purpose; moreover, the production process of many multiple vaccines is simple and the production technology Rough, causing some products to be polluted, the titer of some antigens in the antigen composition is reduced, and cannot meet...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of Newcastle disease single vaccine

[0031] The chicken Newcastle disease adopts the LaSota strain, and the virulent virus of the chicken Newcastle disease virus is preferably the virulent Beijing strain of chicken Newcastle disease virus (CVCC AVI611 strain). The standards and storage methods of the virus seeds are the same as those specified in Item 1 of the "Regulations for the manufacture and inspection of chicken Newcastle disease low-virulence live vaccines" in the "Regulations of the People's Republic of China on Veterinary Biological Products" (hereinafter referred to as "the regulations"). The chickens used in the safety test and inspection of the double vaccine are all SPF chickens or susceptible chickens.

[0032]Use the LaSota strain as the virus seed, inoculate 10-day-old SPF chicken embryos, discard the dead chicken embryos within 24 hours, harvest the dead chicken embryo fluid with obvious lesions 72-120 hours after inoculat...

Embodiment 2

[0033] Embodiment 2: the preparation of infectious bronchitis single vaccine H120 strain or H52 strain

[0034] H120 strain or H52 strain is selected for manufacture of this product, and standard virulent M41 strain is used for inspection, both of which are provided by the applicant Ruipu Company. The chickens used in the safety test and inspection of the double vaccine are all SPF chickens or susceptible chickens.

[0035] Among them, the test uses the standard strong poison M 41 Strain virulence and virus seed standards are as follows:

[0036] Viral content M 41 For 1-7 day-old healthy susceptible chicks, 1-2 drops of 10-fold diluted strong poison can be used to observe for 10 days, and at least 80% of the chicks should have the typical symptoms of chicken infectious bronchitis.

[0037] For the inspection standards of seed virus for vaccines (virus content, safety, immunogenicity, purity, and specificity of the basic seed generation), see the protocol P212.

[0038] Th...

Embodiment 3

[0044] Embodiment 3: the preparation of new branch dual live vaccine

[0045] The virus strain used in the manufacture of this product is the attenuated Newcastle disease strain LaSota strain, and the strong virus used in the efficacy test is the virulent Beijing strain of chicken Newcastle disease virus (CVCC AVI611 strain). The infectious bronchitis virus strain used for manufacturing is H120 strain or H52 strain, and the standard virulent M41 strain is used for inspection. The above-mentioned strains should meet the relevant virus requirements in the production and inspection regulations of chicken Newcastle disease low-virulence live vaccine and chicken infectious bronchitis live vaccine. Standards 1.2 and 1.3 of the species section.

[0046] For semi-finished and finished product inspection standards, see Regulations P-224-226.

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PUM

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Abstract

The invention relates to a method for producing a combined vaccine, in particular to a method for producing a combined vaccine against Newcastle disease and infective bronchitis. The method comprises that: before culture in the same embryo, purity of breeding virus of the Newcastle disease and the infective bronchitis is improved; and during culturing in the same embryo, concentrations of two antigens in the combined vaccine against the Newcastle disease and the infective bronchitis are adjusted to reach reasonable combination. The vaccine prepared by the method is vaccinated with chicken flocks to generate ND and IB immunity without any interference. Furthermore, the vaccine strains are reasonably matched to effectively solve the problem of serious interference in simultaneous vaccination of the Newcastle disease living vaccine and infective bronchitis living vaccine, improve immunization quality and reduce the number of immunization.

Description

technical field [0001] The invention relates to a production method of a dual vaccine, specifically, a production process of a dual vaccine for Newcastle disease and infectious bronchitis, belonging to the field of bioengineering. Background technique [0002] Newcastle disease virus (Newcastledisease virus, NDV) is also known as Asian fowl plague virus, pseudo fowl plague virus or avian pneumoencephalitis virus. The position in virus taxonomy belongs to Paramyxoviridae (Paramyxoviridae), a species in Paramyxovirus genus (Paramyxovirus). The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly among the infested flocks, and the virulent strain can destroy the whole flock of chickens. Attenuated strains only cause respiratory tract infection and decreased egg production in chickens, but they can recover quickly. Humans can develop conjunctivitis or lymphadenitis due to contact with sick birds and live virus vaccines, but recover quickly. Newcastle dis...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61K39/17A61K39/215
Inventor 郁宏伟杨保收
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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