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Method for analyzing fatty acid chemical composition in cortex periplocae radicis

A technology for chemical components and fatty acids, which is applied in the field of analysis of chemical components of fatty acids in Persica sativa by GC-MS technology, and can solve the problems of extraction and analysis of unseen fatty acids

Active Publication Date: 2012-06-06
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports about the extraction and analysis of its fatty acid components at home and abroad.

Method used

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  • Method for analyzing fatty acid chemical composition in cortex periplocae radicis
  • Method for analyzing fatty acid chemical composition in cortex periplocae radicis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1 Instruments, materials and reagents

[0020] 1.1 Instruments GC-MS coupled instrument (SHIMADZU 6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German Satorious Company).

[0021] 1.2 Medicinal materials and reagents Cinnamon bark, purchased from Shenyang Medicinal Materials Company, was identified as authentic by Professor Sun Qishi, Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University. All reagents used were of analytical grade.

[0022] 2 Experimental part

[0023] 2.1 Fatty acid extraction: 100 g of Cinnamon bark is crushed and extracted with petroleum ether (60-90° C.) under reflux for 8 hours, filtered, concentrated under reduced pressure, and evaporated to dry the solvent to obtain fatty acid.

[0024] 2.2 Methyl esterification of samples Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60°C water bath for 15 minutes, then add 2 mL of boron trifluoride-diethyl...

Embodiment 2

[0035] 1 Instruments, materials and reagents

[0036]1.1 Instruments GC-MS coupled instrument (SHIMADZU 6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German Satorious Company).

[0037] 1.2 Medicinal materials and reagents Cinnamon bark, purchased from Shenyang Medicinal Materials Company, was identified as authentic by Professor Sun Qishi, Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University. All reagents used were of analytical grade.

[0038] 2 Experimental part

[0039] 2.1 Fatty acid extraction: 100 g of Cinnamomum officinalis medicinal material was crushed and extracted with petroleum ether (60°C-90°C) for 6 hours under reflux, filtered, concentrated under reduced pressure, and evaporated to dry the solvent to obtain fatty acid.

[0040] 2.2 Methyl esterification of samples Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60°C water bath for 15 minutes, then add 2 mL ...

Embodiment 3

[0047] 1 Instruments, materials and reagents

[0048] 1.1 Instruments GC-MS coupled instrument (SHIMADZU 6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German Satorious Company).

[0049] 1.2 Medicinal materials and reagents Cinnamon bark, purchased from Shenyang Medicinal Materials Company, was identified as authentic by Professor Sun Qishi, Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University. All reagents used were of analytical grade.

[0050] 2 Experimental part

[0051] 2.1 Fatty acid extraction: Take 100 g of Cinnamomum officinalis medicinal material, pulverize and extract with petroleum ether (60°C-90°C) under reflux for 10 hours, filter, concentrate under reduced pressure, and evaporate the solvent to obtain fatty acid.

[0052] 2.2 Methyl esterification of samples Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60°C water bath for 15 minutes, then add 2 mL of boro...

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Abstract

The invention provides a method for analyzing chemical components of fatty acid in cortex periplocae radicis. The method includes the following steps: (a) the fatty acid is extracted from the cortex periplocae radicis; (b) methyl ester is performed for the fatty acid; (c) the methyl esterifed fatty acid is detected by a GC-MS combination method, wherein the chromatographic condition for GC-MS combination is as follows: the chromatographic column is a quartz capillary chromatographic column; the carrier gas is a high-purity helium gas; the temperature of an introduction port is 230-270 DEG C; the split ratio is 8:1-12:1; and the flow speed is 0.8-1.2mL.min<-1>. The mass spectrum condition is as follows: the ion source is an EI ion source; the temperature of a tetrode is 120-170 DEG C; the temperature of a transmission line is 270-310 DEG C; the voltage of a multiplier tube is 1800-2200V; and the temperature of a GC-MS interface is 210-250 DEG C. Being tested by a methodology, the method is accurate and reliable and can be used for analyzing the chemical components of fatty acid in cortex peripolcae radicis.

Description

technical field [0001] The invention relates to a method for analyzing the chemical components of fatty acids in Persica persicae, in particular to a method for analyzing the chemical components of fatty acids in Persia persicae by using GC-MS combined technology. Background technique [0002] Periploca speium (Periploca speium), also known as North Acanthopanax speium, Sepium bark, is the dry root bark of Periploca sepium Bge, a plant of the Radixaceae family. Persianthus bark tastes pungent, bitter, warm; poisonous, returns to the liver, kidney, and heart meridian, can dispel rheumatism, strengthen bones and muscles, and is used to treat rheumatic arthralgia, soreness of waist and knees, palpitation, shortness of breath, and edema of lower limbs. It is used in modern clinics to treat rheumatoid arthritis, breast cancer, etc.; its total saponins are effective in treating chronic congestive heart failure (Deng Shixian, Wang Decheng, Wang Maode, et al. Cardiotonic effect of d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/02
Inventor 李莉佟玲周水平高钧马洁毕开顺
Owner TIANJIN TASLY PHARMA CO LTD
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